Research Of Mechanism Of Reinforcing Kidney Chinese Herbs To Improve The Treatment Outcome Of In Vitro Fertilization Based On Epigenetics

Objective: In view of epigenetics, to explore the mechanism of reinforcing Kidney Chinese herbs to improve the treatment outcome of in vitro fertilizationMethod: In clinical study, 66 infertile women of kidney yin defficiency undergoing in vitro fertilization-embryo transfer (IVF-ET) were randomized into the test group, treated with ETG and Gn(33 cases), and the control group, treated with Gn only(33 cases). The test group had taken reinforcing Kidney Chinese herbs for 3 months, while the control group hadn’t. Just one month before IVF, all patients of both groups were scraped the endometrium during the midluteal phase to evaluate the DNMT1. The long term protocol was adopted. The syndrome scores of TCM, administration time and number of Gn, thickness and type of endometrium, PI/RI of uterous artery and endometrial blood stream, number of retrieved oocytes, fertilization rate, high quality embryo rate and pregnancy rate were evaluated. In experimental study, 105 Kunming female mice of 8～9 weeks old were randomized into the kidney yin defficiency model group, the kidney yin deficiency test group, and the control group. After the model was established successfully, all the mice were treated with COH. The occtyes of thirty mice were retrieved to evaluate the methylation the imprinted gene of Snrpn and Pegl/Mest. After cohbaiting,the fertilized ovum of the other five mice were retrieved to evaluate the ability of merogenesis and forming the embryo.Results: Symptoms of kidney asthenia of the test group was significantly improved. The level of E2 per occtye, the ratio of high quality occtye and embryo, the clinical pregnancy rate were higher than those of the control group. The difference were statistically significant (P<0.05). The expression of DNMT1 of the endometrium of the test group was higher than that of the control group, the type of endometrium was better than that of the control group, PI and RI of uterous artery and endometrial blood stream of the control group were lower than those of the control group. The difference was statistically significant (P<0.05). The DNA methylation of Snrpn and Pegl/Mest of the kidney yin defficiency model mice occtye were lost. The abnormality was prevented partly in the kidney yin deficiency test group. The number, ability of merogenesis and forming the embryo of fertilized ovum of the kidney yin defficiency model mice were lower than those of the blank mice. The difference was statistically significant (P<0.05).Conclusions: Reinforcing Kidney Chinese herbs combined with western medicine can decrease the dosage of Gn obviously and improve symptoms of kidney asthenia, the quality of oocyte, the ratio of high quality occtye and embryo, the ratio of pregnancy. Reinforcing Kidney Chinese herbs can improve the thickness and type of endometrium, PI and RI of uterous artery, endometrial blood stream, DNMT1 expression of the endometrium. The kidney yin defficiency can make the methylation the imprinted gene of Snrpn and Pegl/Mest lost. It indicates that the abnormality of DNA methylation may be the epigenetic mechanism of the infertility of kidney yin defficiency. Reinforcing Kidney Chinese herbs can prevent the abnormality partly and improve the ability of merogenesis and forming the embryo of the fertilized ovum.That maybe the mechanism of reinforcing Kidney Chinese herbs to cure the infertility.