Research Of Equivalence For "Young Magnolia" And Magnolia Officinalis On The Basis Of Metabolic Frame-work And Preparation Of Fresh Herbs

Magnolia is a traditional Chinese herb medicine, which is the dried parts of stem bark, root bark and branch bark of Magnolia officinalis Rehd.et Wils. or Magnolia officinalis Rehd.et wils.var.biloba Rehd.et Wils.. The severe situation of resource scarcity gradually becomes worse and worse as its broad usage. This thesis is intended to study the process method and resource utilize method primarily basing on the fresh herbs and through the way of plant metabolomics technology to analyze the data which accumulated by metabolism of magnolol and Honokiol in Magnolia. This study brings forward the conception of "Metabolic framework" for the first time, and conduct a comparative research on chemical, pharmacological, toxicological and clinical equivalence of traditional Magnolia and "Young Magnolia", which is intented to explore the high efficiency on the basis of "Metabolic framework" and fresh herbs in conjunction with the research of related resources so as to make the best of Chinese medicine resources.We got the following results from the experiments related to this thesis.(1) We use the magnolol and honokiol as the marker compounds, and prove that the uses of 2 to 5 years old "Young Magnolia" to replace traditional Magnolia as alternative sources of raw materials is feasible.(2) Comprehensively consideration of the technical feasibility of choosing 3-year-old "Young Magnolia" to replace 10 or more years of traditional Magnolia as alternative source of raw materials so as to obtain total magnolol and Honokiol quickly and efficiently.(3) After the separation of gas chromatography and scan by mass spectrometry, we can draw the conclusion that there were very little difference in volatile chemical compositions between both root bark and stem bark of "Young Magnolia",.(4) From the experiment we can draw the conclusion that "Young Magnolia" Sanwu Decotion and traditional Houpu Sanwu Decoction had equivalent medical effects.(5) We found no toxicity in water extracts and alcohol extracts of "Young Magnolia" and traditional Magnolia in the experiment of acute oral toxicity, and the maximum tolerated dose is 200g·kg-1(the original ingredients of dollars).(6) In the clinical observation of Magnolia Bark Sanwu Decotion's medical effects on hot functional constipation of the elder patients, we found similar clinical treatment efficiency for "Young Magnolia" Houpu Sanwu Decoction group and traditional Houpu Sanwu Decoction group and there were no adverse reactions occurred.(7) Further experiments shall be conducted from every aspect on other representative plants such as Macleaya cordata, Macleaya microcarpa, Scutellaria, and Grape etc.. to explain in detail based on "metabolic framework" and fresh herbs of traditional Chinese medicine resources and use patterns of thinking.