Studies On The Chemical Components,biological Activity Of Magnolia Officinalis Rehd.et Wils

Magnolia officinalis Rehd.et Wils.belong to Magnoliaceae of Magnolia.The stem bark of Magnolia officinalis and Magnolia officinalis biloba all called Magnolia officinalis.Most of them distributed in anhui ?the west of zhejiang?jiangxi(lushan)?fujian?the south of hunan?the nourth of guangdong and guangxi.And usuarlly grow in the jungle at an altitude about 1300~1400 meters.Bark,roots,flowers,seeds and shoots all can be used in drug for the treatment of abdominal distention and Pain,dyspepsia,and Phlegm of cough with asthma.Objective:1)To study the chemical constituents of Magnolia officinalis Rehd.et Wils.2)To study the biological activity of the compounds from Magnolia officinalis Rehd.et Wils.3)Study on Phenylethanoid glycosides in vivo pharmacokinetics of Magnoloside A in rats.Methods: Using continuous heating reflux extraction to extract the compositions of Magnolia officinalis and with many methods such as silica gel column chromatography?gel column choromatography ? macroporous resin column chromatography ? reversed-phase chromatography and so on.By using UV spectrum?IR?MS?NMR and other modern spectrum technology to identify the structure of compounds.Magnoloside A is a phenylethanoid glycoside which by construction of gastric ulcer model to observe if whether has prevention function for irritability gastric ulcer.Rats by oral administration and wre intravenously injected with Magnoloside A.With baicalin as the internal standard,using the high-performance liquid chromatography to detection the concentration of drugs in rat plasma at different time.SHIMADZU LC-20 A high-performance liquid chromatograph with a Agilent Zobax SB-C18 column(250*20 mm,5 ?m)by a gradient elution using(A)methanol-(B)water as the mobile phase 0 min(15%A)?15 min(85%A)and the flow rate was 1 mL·min-1.DAS 2.0 software was applied to fit the blood concentration and calculate corresponding pharmacokinetic parameters.Results: Ten compounds were isolated from Magnolia officinalis and their structures were elucidated as magnolol(1)?honokiol(2)?syringin(3)??-sitosterol(4)?daucosterol(5)?quercetin(6)?D(+)-sucrose(7)?1,1'-dibenzene-6',8',9'-trihydroxy-3-allyl-4-O-?-D-Glucopyr anoside(8)?magnoloside A(9)?Magnaldehyde B(10).The rats by oral administration and wre intravenously injected at the concentration of 200 mg·kg-1 and 5 mg·kg-1.The target blood quality concentration show good liner relations within the range of 0.3~100?g·mL-1(r=0.9996),the lower limit of standard curve was 0.3 ?g·mL-1.Intra-and inter-day precisions RSD was less than 7.1% and 12.4%;RE ranged between 3.3% and 5.9%;the recoveries ranged between 83.5% and 99.0%.Ragarding the pharmacokinetic pharmeters of oral: AUC(0-t)was(15.6±7.4)mg·h·L-1;CL was(14.5±6.1)L·h-1·kg-1;Vd was(13.5±2.8)L· kg-1;t1/2 was(1.16±0.8)h and tail intravenous injection :AUC(0-t)was(17.8±9.9)mg·h·L-1;CL was(0.34±0.14)L·h-1·kg-1;Vd was(0.08±0.04)L· kg-1;t1/2 was(0.15±0.03)h.Conclusions:In this study,ten compoudas were isolated from Magnolia officinalis and we established a simple,rapid and accurate method to detection the bolood concentration of Magnoloside A,and reported the in vivo pharmacokinetics of Magnoloside A in rats for the first time.