| Poor reproductive performance by turkey hens compared with chickens is partially due to the early cessation of egg production associated with the onset of incubation behavior. Prolactin (Prl) is involved in the induction and maintenance of incubation behavior in birds, and understanding the regulatory mechanism(s) of Prl gene expression will provide fundamental information to manipulate Prl production for better reproductive performance in turkey hens.;To better understand the regulatory mechanism of Prl gene expression, the turkey Prl gene was isolated from a ;Two regions similar to the binding sites for the transcription factor Pit-1/GHF-1 were found within 2 kb of the 5;In order to identify regulatory elements for turkey Prl gene expression, a rat pituitary-derived tumor cell line, GH;The results of the GH, cell transfection experiment indicated that the Pit-1/GHF-1 transcription factor plays an essential role for turkey Prl gene expression. Three cDNAs encoding turkey Pit-1/GHF-1 isoforms, referred to as tPit-1*, tPit-1... |
