| Objective: A high cholesterol diet induction method was used to prepare atherosclerotic rabbit models,and drug-separated cake moxibustion was used to intervene in atherosclerotic rabbits.Observation was made on changes in blood lipid metabolism and tissue structure of atherosclerotic rabbits with drug-separated cake moxibustion.Effect of cake moxibustion on the diversity of intestinal microflora in atherosclerotic rabbits,and the mechanism of action of FX / FGF15 in regulating bile acid metabolism.Methods: 42 healthy purebred male New Zealand big ear white rabbits were randomly divided into 6 groups: normal group,model group,probiotic group,antibiotic group,medicated cake moxibustion group,medicated cake moxibustion + broad-spectrum antibiotic group.The AS rabbit model was prepared by the high cholesterol diet induction method,and intervention was conducted according to the factors of the subjects.After the intervention,fasting,anesthesia of the ear veins,blood collection from the abdominal aorta,and the animals were sacrificed.Bile,intestinal tissue,small intestine contents,cecum contents,etc.The rabbits in each group were observed for blood lipid levels and HE staining.Intestinal microecology 16 S r DNA analysis technology was used to explore the adjustment effect of cakeseparated moxibustion on AS rabbit intestinal flora.,QPCR detection of liver and intestinal tissue FXR,CYP7A1,FGF15 and other content and expression,enzyme colorimetric method to detect bile acid content.Results:1.HE staining: normal group: each layer of the rabbit aortic wall structure is clearly visible,the lumen intima is smooth,complete,continuous,uniform in thickness,without lipid infiltration,neatly and orderly,without lipid spots;model group : The intima of the middle aorta is obviously thickened,protruding into the cavity,and plaque and lipid deposits can be seen in the wall of the tube,and a large number of foam cells appear;The probiotic group: the intima thickened slightly,and the structure of each layer was still clearly visible.The endothelial cells in the tube wall were continuous.The smooth muscle cells were arranged neatly and regularly,and a few foam cells were formed.Thick,but the structure of each layer is clear,the endothelial cells of the tube wall are continuous,smooth muscle cells are proliferated,and a large number of foam cells are formed;Group 1 moxibustion cake cake: the intima is slightly thickened,the layers are still clearly visible,and the endothelium of the wall is continuous Smooth muscle cells are uniform in thickness,neatly arranged,and foam cells are rare.Group 2 moxibustion cakes: thickened intima,clear layer structure,continuous endothelial cells in the wall,smooth muscle cells hyperplasia,and a small number of foam cells formed.Compared with the normal group,the serum cholesterol and triglyceride levels of the rabbits in each group were higher,and the difference was statistically significant(P <0.01).Compared with the model group,the levels of serum cholesterol and triglyceride in the probiotic group,antibiotic group,medicated cake moxibustion group 1 and medicated cake moxibustion group 2 were significantly reduced(P <0.01).Compared with the antibiotic group,serum cholesterol in the two groups of moxibustion cake moxibustion was significantly reduced(P<0.05).Compared with the two groups of moxibustion cake moxibustion,the serum cholesterol of group 1 moxibustion cake moxibustion was significantly reduced(P <0.05).2.Compared with the blank group,the model group's microbial diversity and uniformity increased slightly,and the blank group's model group had a different colony composition;compared with the model group,the drug-separated cake moxibustion group's flora richness,diversity and uniformity All have increased;compared with the model group,the probiotics group's flora richness,diversity and uniformity have increased;compared with the model group,the antibiotic group and the drug-separated cake moxibustion plus antibiotic group's flora richness and diversity As well as the degree of uniformity decreased;compared with the antibiotic group,the diversity and uniformity of the flora diversity and uniformity of the drugseparated cake moxibustion plus antibiotic group decreased slightly.3.Compared with the blank group,Lachnospiraceae and Firmicutes in the model group are the dominant bacterial species;compared with the model group,Akkermansiaceae in the cake-separated moxibustion group.The relative proportion of strains increased in the medicated cake moxibustion group,and Cyanobacteria,Melainabacteria,and Gastronaerophilales were the dominant strains;compared with the antibiotic group,the medicated cake In the moxibustion plus antibiotics group,Akkermansiaceae accounted for a relatively high proportion.4.Compared with the model group,the expression of CYP7A1,FGF15 m RNA and protein in liver and colon of probiotic group,medicated cake moxibustion group,antibiotic group,medicated cake moxibustion + antibiotic group were significantly increased(P<0.05),while FXRm RNA and Decreased protein expression(P<0.01 or P<0.05),suggesting that cakeparting moxibustion can prevent excessive activation of FXR,and there may be other FXR receptors;compared with cake-parting moxibustion group,cake-parting moxibustion+antibiotic group CYP7A1 FGF15 m RNA and protein table increased slightly(P> 0.05),FXRm RNA and protein expression decreased slightly(P> 0.05),suggesting that cake-separated moxibustion can adjust the FXR / FGF15 axis more and restore the body to normal levels.Compared with the model group,the size of the bile acid pool in the probiotic group,the medicated cake moxibustion group,the antibiotic group,and the medicated cake moxibustion + antibiotic group all increased significantly(P<0.05).Such hardening is important.Conclusion: 1.Medicine cake moxibustion can effectively regulate the level of blood lipids.Antibiotics will weaken the effect of medicine cake moxibustion.At the same time,it can improve the damage of arterial blood vessel walls,inhibit the production of foam cells and the formation of AS plaque.2.Medicinal cake moxibustion of AS plaques in the AS rabbit model,in which the relative proportion of Akkermansiaceae in the intestinal flora has increased compared with the model group,At the same time,Cyanobacteria,Melainabacteria,and Gastranaerophilales in the moxibustion group of medicine-separated cake may be important bacterial groups for anti-AS.The specific mechanism and Increasing the content of Akkermansiaceae in the intestinal flora is related.3.Medicinal moxibustion may increase the expression of CYP7A1 by adjusting the FXR / FGF15 axis to promote bile acid synthesis,increase the size of the bile acid pool,and finally play an anti-atherosclerotic effect. |
PDF Full Text Request