Study On The Quality And Mechanism Of The Antiemetic Action Of Traditional Mongolian Medicine Zhitu Liuwei San

Objective: The traditional Mongolian medicine Zhitu Liuwei San is a good prescription and for relieving vomiting in Mongolian medicine.According to the relevant literature,the research on the Mongolian medicine Zhitu Liuwei San has not yet been in-depth,and the systematic research on the quality research and pharmacological mechanism of Zhitu Liuwei San has not yet been publicly reported.In order to provide experimental basis for the secondary development and promotion of the drug,a study on the pharmacognosy identification,quality control method and pharmacological action mechanism of the Mongolian medicine Zhitu Liuwei San was carried out.Methods: The origin,composition and effect of the Mongolian medicine Zhitu Liuwei San were summarized by searching ancient and modern literatures.The powder identification of Zhitu Liuwei San was carried out by microscopic identification,and the moisture and ash content were carried out according to the Chinese Pharmacopoeia(2015).TLC was used to identify Licorice,Coriander Seed,Simpleleaf Shrub Chastetree Fruit,Fennel and Pyrus ussuriensis Maxin.HPLC method was used to determine the content of glycyrrhizin and glycyrrhizic acid in Zhitu Liuwei San.Cisplatin was used to establish the rat model of heteropexia.The daily intake of kaolin in rats was determined as an indicator of the degree of nausea and vomiting.5-HT synthesis,metabolism of MAOA,transport of SERT and 5-HT3 A receptor substance P and its precursor PPTA and NK1 receptor,dopamine synthesis of TH,suppression(MAOB,DOPAC)and D2 receptor from peripheral(serum,ileum)and central nervous system(medulla oblongata)in rats with cisplatin induced vomiting by ELISA method,Immunohistochemical staining,Western blotting and Real-time PCR.Results:(1)The current clinical use of the traditional Mongolian medicine Zhitu Liuwei San came from the classic Mongolian medicine book,"diagnosis and treatment of the Ming Medical Code" in 8th century,"Xingarri Rukeba",and the two single herbs in the prescription were used as substitutes.(2)The components of the mixture of Zhitu Liuwei San were separated from other components of monopharmaceuticals.The powder microscopic characteristics were different from those of other five drugs without Coriander Seed.TLC method was used to identify Licorice,Coriander Seed,Simpleleaf Shrub Chastetree Fruit,Fennel and Pyrus ussuriensis Maxin.in Zhitu Liuwei San.The water content is 6.89%~7.75%;the ash content is 4.06%~4.40%;the acid insoluble ash is 1.24%~1.44%;the water-soluble extract is 26.5236% ~ 27.3679%;the alcohol-soluble extract is 14.45% ~ 14.56%;the ether extract is 0.05% ~ 0.06%;the heavy metal lead is < 0.01?g/kg;the mercury content is 2.60 mg / kg,chromium 2.41 mg / kg,cadmium 1.194?g/kg,arsenic 0.20 mg / kg;The linear range of liquiritin and glycyrrhizic acid were 0.06 ~ 3.00?g?0.0784 ~ 0.3920?g.The average recovery were 99.20%,100.26%.The content of glycyrrhizin and glycyrrhizin in 10 batches were determined as follows: the content of glycyrrhizin was 0.552 mg/g~1.365 mg/g and the content of glycyrrhizic acid was 1.8744 mg /g ~ 2.582 mg /g.(3)In this study,the rat model of ectopia was successfully made with cisplatin.The intake of kaolin was significantly increased every 24 h(24 h,48 h,72 h)compared with the normal group.The intake of kaolin in Zhitu Liuwei San group and ondansetron group was significantly lower than that in the control group.The results showed that both Zhitu Liuwei San and ondansetron could inhibit the behavior of heteropexia in rats.The treatment of Zhitu Liuwei San for 24 h and 72 h could decrease the level of TPH in ileum and medulla oblongata of rats,and up-regulate the expression of SERT m RNA to inhibit the synthesis of 5-HT,promote the transport and metabolism of 5-HT,up-regulate the expression of 5-HT3 A receptor m RNA and protein,and achieve antiemetic effect by regulating the expression of 5-HT3 A receptor m RNA and protein up and down.The treatment of Zhitu Liuwei San for 24 h and 72 h could decrease the level of TH in ileum and medulla oblongata of rats,DA synthesis was inhibited,expression of m RNA and protein of D2 receptor were down-regulated.The treatment of Zhitu Liuwei San for 24 h and 72 h could reduce the level of substance P in ileum and medulla oblongata of rats,m RNA and protein expression of NK1 receptor were down-regulated.Conclusion: The current clinical use of the traditional Mongolian medicine Zhitu Liuwei San came from the classic Mongolian medicine book-"diagnosis and treatment of the Ming Medical Code" in 8th century.The results of the quality analysis of Zhitu Liuwei San showed that the method has high precision,stability and reproducibility,and can be used as a quality evaluation method for quality control of the drug.The study on the mechanism of antiemetic mechanism showed that Zhitu Liuwei San had a certain curative effect on nausea and vomiting induced by cisplatin,which was closely related to the effects of many targets such as 5-HT,substance P and DA.