A Study On The Relationship Between SNP Of TP53 And Other Genes And Cervical Cancer Susceptibility

Section1 The influence of TP53 Arg72 Pro on the susceptibility of cervical cancer in Chinese han womenSubject:In a case-control study,clinical samples and data of cervical cancer patients and control patients were collected to investigate the influence of single nucleotide polymorphisms on the susceptibility to cervical cancer.In fact,the genes included in the study include TP53,TNRC9,2q35-rs13387042,FGFR2,and MAP3K1,which are intended to provide molecular theoretical guidance and new ideas for the prevention and prognosis of cervical cancer.Methods:A systematic search of the data.The databases searched include Pubmed,Springer Link,Ovid,Chinese biomedical database,Chinese National Knowledge Infrastructure and Chinese Wanfang Database.The cut-off year for these studies is March 2014.The search terms were TP53,P53,single nucleotide polymorphism,and cervical cancer.Retrospective studies will be artificially selected to determine whether they meet the admission criteria.Then the data that meet the inclusion criteria are extracted and analyzed.To study the effect of TP53 gene Arg72 Pro polymorphism on cervical cancer susceptibility in Chinese population.Results:Pro/Pro genotypes were significantly associated with the risk of developing cervical cancer in hospital-derived populations(Pro/Pro vs.Arg/Pro+Arg/Arg: OR=1.55,95% CI: 1.20-1.99;Pro/ Pro vs.Arg/Pro: OR=1.65,95% CI: 1.26-2.16).However,in the more sensitive model,fixed effect model and random effect model,there was no obvious statistical difference.No substantial change has occurred in the combined OR,which indicates that the result is stable and highly reliable.Conclusion:The polymorphism at the 72 locus of TP53 gene is closely related to the risk of cervical cancer in Chinese population,especially the Han nationality.The research of other ethnic groups is still limited.It is necessary to carry out a large sample study and extensively discuss the relationship between the TP53 gene Arg72 Pro polymorphism and the risk of cervical cancer,which will greatly help verify the summary of published papers.Section2 Screening of SNPS related to cervical cancerSubject:Complex genetic disease is a disease caused by a combination of genetic and environmental factors.Cervical cancer is a disease caused by genes and viruses.Cervical cancer caused by genes can be inherited.At present,439 SNPS related to human diseases have been identified by genome-wide association analysis.Cervical cancer is a polygenic genetic predisposition disease.We intend to screen the SNP locus of cervical cancer related genes in the experimental group through GWAS and compare it with the control group to obtain the allele of variation.Methods:The case group came from January 2015 to December 2016 in our hospital and a tumor hospital in Beijing and was diagnosed as 300 cases of cervical cancer by pathology.A total of 300 healthy women in our hospital were selected as control groups during the same period.Results:The inclusion criteria in the control group were no positive results,and no previous history of cancer was found.Parallel cervical cytology or colposcopy/biopsy excluded patients with precancerous lesions and cervical cancer.Genome-wide association analysis was performed between the experimental group and the control group to find SNPs related to the occurrence and development of cervical cancer.Conclusion:A total of 6 S N P s(rs 1042522,rs 3083662,rs 13387042,rs 2981582,rs1219648)sites were selected for screening.A total of 5 genes were involved,of which the FGFR2 gene detected two SNP sites.Section3 The influence of SNP on the susceptibility of cervical cancer in Chinese han womenSubject:To investigate whether the single nucleotide polymorphism in selected sites affects the risk of cervical cancer in some Chinese Han women by comparing six screening sites in cervical cancer and healthy controls(rs1042522,rs3803662,rs13387042,rs2981582 The allele frequencies and genotype frequencies of rs1219648 and rs889312 were used to evaluate the role of the above candidate sites in the risk of cervical cancer in some Chinese Han women to study the molecular mechanisms of the pathogenesis and progression of cervical cancer and to make rapid and effective The targeted gene chip enables rapid screening of potential patients and integrates diagnosis,detection and treatment to establish a targeted platform for the prevention and treatment of cervical cancer.Methods:In this study,a case-control study was conducted to collect clinical specimens and data from patients with cervical cancer and control patients.From the point of view of genetic polymorphisms,the effect of single nucleotide polymorphisms on cervical cancer susceptibility was explored.In fact,the genes included in the study were TP53,TNRC9,2q35-rs13387042,FGFR2,and MAP3K1,which are intended to provide molecular theoretical guidance and new ideas for the prevention and prognosis of cervical cancer.Results:(1)Among the 6 genes screened by GWAS,only the rs532522 gene of TP53 showed differences in gene frequency and distribution.Among other selected genes,there was no significant difference in gene frequency and gene distribution between the experimental group and the control group.(2)The risk of cervical cancer in people with CG genotype was higher than that in patients with CC and GG genotypes.The OR(95% CI)was 1.42(1.13-1.54).(3)After detection,the frequencies of the three genotypes CC,TT and CT of rs3803662 polymorphism in cervical cancer were 20%,41% and 39%,respectively.In the normal control group,the frequencies were 26%,44%,and 30%,respectively.In the cervical cancer group and the normal control group,the distribution was not statistically significant(p>0.05).(4)After testing,the distribution frequencies of three genotypes AA,GG,and AG of rs13387042 polymorphism in cervical cancer were 20%,41%,and 39%,respectively.In the normal control group,the frequencies were 4%,75%,and 21%,respectively.In the cervical cancer group and the normal control group,the distribution was not statistically significant(p>0.05).(5)The frequencies of the three genotypes CC,TT and CT of rs2981582 polymorphism in cervical cancer were 46%,9% and 45%,respectively.In the normal control group,the frequency was 51%,12%,and 37%,respectively.In the cervical cancer group and the normal control group,the distribution was not statistically significant(p>0.05).(6)The distribution frequencies of three genotypes AA,GG and AG of rs1219648 polymorphism in cervical cancer were 38%,13% and 49%,respectively.In the normal control group,the frequencies were 44%,12%,and 44%,respectively.In the cervical cancer group and the normal control group,the distribution was not statistically significant(p>0.05).(7)Using allele A as a reference,the allele had an OR of 0.81(0.66-1.29)in the healthy group and the cervical cancer group.Conclusion:The two groups were compared with each other,p>0.05,so in allele A and allele C,there was no significant difference in the distribution of healthy group and cervical cancer group,and there was no statistical significance.