Studies Of Early Prediction Profile Specific To Cervical Cancer In Uighur Women By Plasma Proteomics

Objective:Protein is a relatively high level product of gene expression control and the information on proteins dynamically altered by disease may be found in blood. In this study, fingerprint features of low abundant plasma proteome specific to early stage cervical squamous cell carcinoma in Uighur women was analyzed by two dimensional liquid phase chromatography. A plasma proteomic model to distinguish patients of early clinical stage tumor from the normal was established, and to provide evidences for further identification of plasma protein markers and establishment of early warning system as well.Methods:48 cases of plasma samples from Uighur women was collected, including 22 cases of cervicitis and 26 cases of early stage cancer at clinical stageâ… -â…¡a; After preparation of low abundant plasma proteome by depletion of high abundant plasma proteins, the proteomic fingerprint features of patients with early stage cancer and cervicitis were studied by separation and analysis of the plasma proteome with two dimensional liquid phase chromatography and to establish a distinguishing model of plasma proteome for patients with early stage cancer subsequent data processed with professional software.Results:The features of low abundant plasma proteome of patients with early stage cancer and cervicitis was studied by two dimensional liquid phase chromatography and a plasma proteome fingerprint model specific to patients with early stage cancer was established. By setting up the protein content difference of more than two times as a standard for quantitative differences,8 peaks with ascending and 1 with descending protein content in patients with early stage of cancer compared to cervicitis were found.Conclusion:The two-dimensional liquid phase chromatography as a high throughput method has a promising application potential in the research of plasma proteomics. The remarkable differences in low abundant plasma proteomes between early stages of cervical squamous cell carcinoma (CSCC) and cervicitis in Uighur women may provide an important evidence for the identification of plasma protein markers of the cancer development and early diagnosis in the future.