Nucleotide Variation In ATG4A And Susceptibility To Cervical Cancer In Southwestern Chinese Women

Objective: The aim of this study was to investigate whether variants in autophagy genes contribute effects of cervical cancer,whether single nucleotide polymorphism(SNP)of ATG4 A have significant relationship with cervical cancer and contribute to HPV infection in the population of Southwestern China.Design and Methods: A total of 542 samples were collected and analyzed for DNA extraction and genotyping.Stratified analysis used to test the correlation between variant genotype of ATG4 A and the risk of cervical cancer.The single SNP and SNP-SNP interaction association analyses with HPV infection were evaluated in sample of cancer case and healthy control.Results: SNPs of ATG4 A,contain rs4036579,rs5973822,rs807181,rs807182 and rs807183 have significant relationship with cervical cancer risk(p<0.05).Meanwhile,rs807181 indicate that variant C allele is associated with lower risk of cervical cancer(p=0.0051,OR=0.596,95% CI 0.403 to 0.821).For rs807183,variant A allele increase risk(p<0.0001,OR=1.573,95% CI 1.305 to 2.085).Stratified analysis suggests that the homozygous variant genotype could decrease the risk of cervical cancer and due regard should be paid to the susceptibility of HPV involved in cervical cancer.The interaction between rs807181 and rs807183,was associated with HPV infection in case and control behind multiple testing corrections(combined p=2.00 × 10-3/3.22× 10-2,respectively).Conclusions: This research dedicate that single nucleotide polymorphisms(SNPs)in an autophagy gene exhibited a significant relationship with the risk of cervical cancer.Indeed,the interactions of ATG4 A SNPs contribute to HPV infection in the population of Southwestern China.Variants may be direct inducers and intermediates in different modalities of autophagy associated with ATG4 A.