The Study Of Biosensors And Biomolecule Logic Devices Based On Nanomaterial

With the rapid development of micro-nano materials,a variety of excellent performance of micro-nanomaterials have been developed,and because of its unique optics,electricity,thermal,chemical and mechanical properties,these materials can be widely used and play a vital role in military,civilian and medical areas.In recent years,micro-nano materials have shown a surprising prospect in biological detection and molecular logic gateand become one of the research hot spots in the field of electroanalytical chemistry and nano-materials.Although the development of micro-nano materials in these areas hasmade some progress,it also faces many shortcomings and challengesin practical application.This work starts with the synthesis of a variety of highly efficient micro-nanomaterials and study its progress in the field of electroanalytical chemistry.The main contents of this paper are divided into two parts.In the first part,the application of the synthesis and assembly of micro-nano materials as the platform to achieve its application in the field of biosensingwith the electrochemical method.In the second part,the application of the synthesis of micro-nanomaterials as a platform combined with fluorescence resonance energy transfer toachieve the development of molecular logic operation.The results are as follows:[1] Zn O-Cu O composite nanomaterial with three-dimensional porous structure was prepared by coaxial electrospinning method.The Zn O-Cu Ocomposite nanomaterial with different thickness was prepared by controlling the time of coaxial electrospinning with FTO electrode as carrier.The results show that the prepared three-dimensional porous Zn O-Cu O electrode has a high sensitivity to glucose detection under the condition of free enzyme,and its sensitivity is 3066.4?Am M-1cm-2.The detection range is 0.47 ?m-1.6 m M,and the lowest detection limit is 0.21 m M.[2] The gold nanorods with different aspect ratio were synthesized by hydrothermal method,and the gold nanoparticles were prepared by the same method as the comparative experiment.The glassy carbon electrode was used as the carrier,and the prepared gold nanorodswith different aspect ratio that assembled on the surface of electrodes were used to detect the liver cancer markers with high sensitivityunder the condition of enzyme-free and labeling-free.The detect range was0.1-200 ng/ml and the lowest detection limit was 0.04 ng/ml.[3] First,the gold nanoparticles were synthesized by hydrothermal method.Secondly,by studying the connection of poly A base modified DNA sequence with gold nanoparticles under different p H conditions,it was confirmed that poly A-DNA sequences can be rapidly connected to gold nanoparticles at p H=3.The DNA sequence on the gold nanoparticle could hybridize with another fluorescent modified DNA sequence and regarded as the reaction platform.Then,the different DNA sequenceswere used as the input signal to realize a series of advanced molecular logic gates: half adder,halfsubtractor,2-1 encoder and 4-2 encoder.[4] First,the grapheneoxide nanomaterial was firstsynthesized,followed by the use of the special properties of graphene oxide thatcould adsorb single-stranded DNA sequence through ?-? bond and quench some fluorescent groups,the combination of the fluorescent modified single-stranded DNA sequence and graphene oxidecould be regarded as the reaction platform and implemented a variety of advanced binary molecular logic gates,including full adder,fullsubtractor and majority logic gate.[5] Through the summary and further study of the previous work,the use of graphene and DNA binding structure as reaction substrate to further realize the advanced logic gate,that is,parity checker and prime discriminator.[6] By combining GO and single-stranded DNA,and through the hybridization between DNA,this work successfully realized reversible advanced logic gate in asimple and efficient way with repeatable realization.[7] Through further research to overcome the shortcomings of the binary molecular logic gate circuit and show the versatility of the combinationgraphene oxide and fluorescent modified DNA,we also designed and successfully realized the ternary molecular logic gate circuit,including ternary OR and ternary INHIBIT logic gate.