Difference Expression In Various Organs Of Camellia Pubipetala And Molecular Authentication On C. Pubipetala And Berchemia Lineata

With the rapid growth of the international market and the increasing demand for quality Chinese herbal medicines,the correct identification of Chinese herbal medicines is a key factor in ensuring its safe,effective and fair trade.Molecular identification techniques provide a reliable and powerful tool for different classification levels,including identification of species,genera,species,variants and cultivars.With the initiative of DNA barcoding,it has become a hot topic in recent years of biodiversity research.The standard DNA barcode fragments can be accurate and rapid for identification and identification of species.As the DNA extraction and sequencing costs are gradually reduced,more molecular markers of bar code identification methods can be found and promoted,more molecular markers of the bar code identification method can be found and promoted,the number of DNA bar code laboratory,DNA molecular identification work will be more convenient to carry out.In this study,as research object by B.lineata and C.pubipetala,The molecular identification system of B.lineata and C.pubipetala was established.Berchemia lineata is a rat or a dwarf shrub with swelling and detoxification,bleeding and analgesic,Qufeng dehumidification and other effects.In this study,we used ITS2,psbA-trnH and rbcL as the identificationsystem,and examined a total of 56 plant samples from seven Berchemia.The identification efficiency rate of ITS2 was found to be 100% at the species level.However,through the analysis of the pairwise distance,the varieties B.lineata and Berchemia.polyphylla var.leioclada was a distance value of zero,showed that the ITS2 region cannot differentiate the varieties of these species.Therefore,as indicated by the high degree of sequence variation,the pairwise distance analysis was proven useful in Berchemia identification but only up to the species level.C.pubipetala is an evergreen shrubs or small trees,which belong the Theaceae,mainly production in Guangxi of China.There are "tea family queen","plant giant panda" reputation,it is the world's famous ornamental plants,is also extremely valuable medicinal plants.C.pubipetala was listed as a national key level to protect plants.This paper systematically summarizes the ways and development of molecular authentication technology of C.sinensis,It was obtained some research progress in the chemical composition,pharmacology,tissue culture,molecular biology,physiology,agronomic cultivation and protection of biology,and resource development and so on.By querying NCBI and other databases,there is no data or genome data of Camellia ternata,which makes the lack of genetic background research on C.pubipetala and can not obtain the data support from high throughput sequencing.The existing studies are limited to the few genes obtained by homologous cloning.Based on the DNA barcode identification of C.pubipetala as the test material,RNA-seq technology was used to establish the transcriptome database of C.pubipetala at the RNA level,and the characteristics of two transcripts of C.pubipetala flower and its leaf were obtained.The two groups of transcripts were compared and analyzed,and the characteristics of the expression of C.pubipetala were studied systematically.In this study,a total of 90960 Unigene were obtained,a total of 60233 ofUnigene can be noted by Nr,GO,KOG,KEGG and other database.It accounted for 66.2% of all Unigenes,including the GO database of three major categories,45 small classes,26 categories of COG database,and 279 metabolic pathways of KEGG database.It was explicited the expression characteristics differences of the flower and the leaf of C.pubipetala,and a database platform was established successfully for the Camellia transcriptome.In conclusion,In this study,with DNA barcode identification technology,the candidate barcodes of B.lineata and C.pubipetala was evaluated through the study on DNA barcoding technique.At the same time,for a further comparative analysis of two transcripts of C.pubipetala,using the transcriptome database platform of Camellia,by mining the camellia flavonoid and terpenoid secondary metabolism during the synthesis of the key gene,to reveal the physiological process for the subsequent participation of camellia,the expression levels of genes related to drug synthesis,provide data support to carry out regulation of secondary metabolites of C.nitidissim.