Investigation On Chemical Constituents Of Bechemia Lineata(L.)DC.and Its Inhibitory Activities On CYP450 Enzyme

In this thesis,the ultra high performance liquid chromatography(UPLC)and quadrupole time-of-flight mass spectrometry(QTOF-MS)technologies were used to systematically study the chemical constituents of Berchemia lineata(L.)DC.(BL),and the inhibition activity on CYP450 enzyme.The main research reports was as follows:Firstly,the crude extract of BL was prepared and the UPLC-QTOF-MS/MS analysis method was established and optimized.The validation results of the UPLC method demonstrated that the developed method possessed desirable precision(RSD<1.79%),reproducibility(RSD<4.05%)and accuracy(RSD<1.80%).A total of 35 compounds of BL crude extract were detected,and 25 of which including 6 naphthopyrones,10 flavonoids,2 phenolic acids,2 phenols,4 fatty acids and 1 quinone were unambiguously or tentatively identified by comparing their retention time and accurate mass measurement with reference compounds and data in literature.Naphthopyrones were first detected in BL and labeled in the chromatogram.Secondly,the chemical quality of BL were performed.The UPLC fingerprints combined with the chemometrics methods and antioxidant activities were carried out to evaluate the chemical quality of 11 batches of BL.A total of 26 common peaks were matched in the chromatogram within 36 min in a run.And 11 batches of BL from different sources were classified into two categories by clustering analysis and principal component analysis.The in vitro antioxidant study showed that the antioxidant capacity of BL was strongly negative correlated with the amount of naphthalene pyridones and flavonoids per unit mass.Finally,the inhibitory activity of BL on CYP450 Enzyme was carried out.Results showed that the BL extract showed weak inhibitory effect on human CYP3A4.The further metabolism investigation of the main component in the BL extract showed that the phase I metabolic pathway existed.And the hydroxylation product was found as 10-hydroxy-rubrofusarin-6-O-?-L-rhamnosyl-(1-6)-O-?-D-glucopyransi-de by mass fragments.The results could be used to guide the rational utilization of BL in clinic and provided scientific basis for in vivo and in vitro metabolic study of BL.