| Objective:(1)To establish the rabbit model of cervical spondylosis,to observe the change of IVD,ligamentum flavum,etc.(2)To measure the stress distribution of rabbit cervical,(3)To collect the ligamentum flavum of patients who were received the cervical operation using posterior approach.Methods:(1)Normal male rabbits(weighted 2,0-2.2kg)were divided into two groups randomly.The experimental group were fixed with spring on the spinous process of C6,C7,and the control group were fixed with breaked spring.3 months after the operation,all the specimens were engaged both in iconographic examination(X-ray,MRI),morphological examination(the light microscope,orcein stained examination)and in the apoptosis examination.Integral absorbance analysis was used to evaluate the immunohistochemistry outcome.(2)C1-Tl specimen of normal male rabbits were mesured stress with strain gauge.(3)The ligamentum flavum of patients were examined by light microscope,immunohistochemistry and apoptosis.Result:(1)In iconographic examination:the angle and signal of spinal cord is abnormal.Under the light microscope:the volum of nucleus pulposus was decreased and fissure was founded,the sturcture of anulus fibrosus was distroyed and fissure was founded in collegen fiber also,the endplate was calcified and cell was pyknosis and dissolved.The arrangement of ligamentum flavum was disorded.The experimental ligamentum flavum were orcein stained strongly and the number of cell is increased.Apoptosis was founded in experimental spine cord.(P<0.05)(2)There was no significant in different segment when flexed from 10 to 20°,and was significant in C5,C6 when flexed from 30 to 40°(P<0.05).(3)The arrangement of elastic fiber was disordered and disrupted in degenerated ligamentum flavum.Conclusion:(1)The rabbit cervical in micro-stress was degenerated,cell in the spinal cord was apoptosis.The changes in the ligamentum flavum were similar with that in humen.(2)The stress in verticle is differ in different segment when cervical moving. |
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