| Objective:Comparative studie between D.aurantiacum Rchb. f.var.denneanum (Kerr) Z.H.Tsi, D.nobile Lindl., D.chrysotoxum Lindl., and D.fimbriatum Hook.. Also investigate the stem, leaf, flower of D.aurantiacum Rchb. f.var.denneanum (Kerr) Z.H.Tsi. To found the biological genetic relationship of D.aurantiacum Rchb. f.var.denneanum (Kerr) Z.H.Tsi and those species, and provide the reference for comprehensive utilization of D.aurantiacum Rchb.; Isolation and identification of the chemical composition of the D.aurantiacum Rchb., to clarify the type of chemical composition in it and to provide a basis for its quality control. To investigate preparation process of formula particles of D.aurantiacum Rchb. to expand the range of applications and increase the income of the growers, improve planting initiative, to bring greater economic benefits.To laid the foundation for the sustainable use and rational development of D.aurantiacum Rchb.Method:The use of TLC, UV-Vis, HPLC, GC and other modern analytical techniques, from Four aspects of different levels to investigate the chemicalc onstituents of4species of Dendrobiumand the stem, leaf, flower of D.aurantiacum.Rchb. To isolated and identified the chemical constituents of D.aurantiacum.Rchb., and to determine the content of it. To investigate preparation process of formula particles of D.aurantiacum Rchb. from the extraction, drying process, materials selection, molding process, Etc.Result:contrast research of Four DendrobiumD.aurantiacum Rchb.contains gigantol, but not the detection Dendrobine and Erianin. The detectable result of TLC and HPLC complement each other, and visualize the similarities and differences of chemical composition of four dendrobium.the comparative analysis result of fat-soluble ingredients by GC-MS has verified that four Dendrobium with the same ingredients. The same chromatographic information to show that they have the same chemical composition, as the same plant that may have a similar effect. Specific chromatography show that the differences in the chemical composition,and should be pay attention that whether they have the the same functions and indications. Comparison results prompte that the chemical constituents study of D.aurantiacum Rchb. and D.fimbriatum Hook.having a greater potential.The result of Content Determination show that D.nobile Lindl. and D.fimbriatum Hook.have similar content of carbohydate components, while D.chrysotoxum Lindl. and D.aurantiacum Rchb. are similar., speculated to have similar efficacy in immune regulation, anti-tumor and regulation of blood sugar, but the dose should be different. The content of gigantol is quite different in different Dendrobium, D.aurantiacum.Rchb. and D.fimbriatum Hook.has the high content about220μg/g, suggesting that it has a certain value in the anti-diabetic cataract.Test results of free sugars by GC-MS show that rhamnose, arabinose. mannose and glucose were detected, but not lactose. D.nobile Lindl. also found to contain galactose.Comparative study of the stem, leaf, flower of D.aurantiacum Rchb. f.var.denneanum (Kerr) Z.H.Tsi.The comparison results of TLC show that the chromatography spots of D.aurantiacum.Rchb. are abundant. HPLC comparison results show that the leaf and flower have more abundant of chromatographic information, speculated that it may have the same chemical composition and similar efficacy. The results of GC-MS show that there are four common components in stem, leaf and flower. Stem have8.13common components with leaf and flower. Stem has higher content of alkanes, but leaf and flower with higher levels of fatty acids and esters, and the component types of leaf and flower significantly more than the stem.Prompt that chemical composition in leaf has a certain significance, and can provide the basis for its comprehensive utilization.The the content determination of carbohydrate show that the content of reducing sugar in stem is lower than in leaf, but the other are higher than it. The determination results suggest that leaf could has a certain research value about pharmacological activity of polysaccharideIsolation and identification of chemical composition and content determination of D.aurantiacum.Rchb.To separation and purification the chemical composition of D.aurantiacum.Rchb.. a total of17compounds were identified. Among those, coniferaldehyde, vanillic acid, vanillin, bis(2-ethylhexyl)phthalate, syringaldehyde,5-hydromethylfuraldehyde,4,5-dihydroxy-2-methoxy-9,10-dihydrophenanthrene,5-acetoxymethy lfuraldehyde, syringic acid and andemodin are first separated from D.aurantiacum Rchb.The content determination result by HPLC of4,5-dihydroxy-2-methoxy-9,10-di-hydrophenanthrene in D.aurantiacum.Rchb. show that the content is only17.42μg/g. The result of content determination to provide a reference of quality control of D.aurantiacum.Rchb. The separation and purification of4,5-dihydroxy-2-methoxy-9,10-dihydrophenanthrene and naringenin to provide some basis of anti-cancer activity of D.aurantiacum.Rchb. The separation and purification of gigantol and syringic acid to providing a reference that D.aurantiacum.Rchb. might have pharmacological activity in the anti-diabetic cataract.Investigate preparation process of formula particles of D.aurantiacum Rchb.Through experimental studies to determine the preparation process of formula particles, the preparation process is stable, viable, and can be used for industrial production. The content determination result showed that the transfer rate of target component is82.8%, indicating that the preparation process to meet the requirements of industrial production, to expand the scope of the market and laid the foundation for the rational development of D.aurantiacum Rchb.Conclusion:Through comparative studies to provide some reference for medicinal value and utilization of D.aurantiacum Rchb. f.var.denneanum (Kerr) Z.H.Tsi. The separation of chemical composition to provide some support the material foundation and pharmacological activity of it. The content determination provide some basis for quality control of it. The preparation technology of formula particles to expand the application of the market and laid the foundation for the rational development of it. |
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