| 1. Comparative study of8kinds of postoperative abdominal adhesion modelsObjective:To study the features and application of8kinds of rat model of postoperative abdominal adhesion. Methods:180Wistar rats were randomly divided into9groups of20animals in each, including sham operation group and8model groups. The survival of each group was registered, survivors were sacrificed on the seventh post-operative day, and the degree of peritoneal adhesion was evaluated by Nair adhesion scale, and the adhesion tissues were examined by histopathology. Results:The survival rates of Group E, Group D and Group I were significantly higher than other model groups. The scores of adhesion of Group G, Group H and Group1were higher than other groups. Conclusion:Duodenum clamped using hemostat is an ideal method for postoperative abdominal adhesion rat model.2. Duodenum clamping trauma induces significant postoperative intraperitonealObjective:To investigate the histological and morphological changes in the first14postoperative days on a rat intraperitoneal adhesions model induced by duodenum clamping trauma. Method:The rat model of postoperative intraperitoneal adhesions was established in48male Wistar rats by laparotomy, followed by the duodenum clamping trauma. Rats were sacrificed immediately after the operation (0day), and then on1st,3rd,5th,7th and14th day, respectively. The intraperitoneal adhesions were assessed macroscopically. Histopathology and immunohistochemistry were performed to evaluate the adhesion tissues. In addition, the changes of the mesothelium covering the surgical sites were examined by scanning electron microscopy. Results:The extent and tenacity of intraperitoneal adhesions reached their peaks on3rd and5th days, respectively. Histopathological examination showed that all rats developed inflammatory responses at the clamped sites of duodenum, which was most prominent on1st day. The scores of fibrosis and vascular proliferation increased slowly from3rd to5th day. Myofibroblasts proliferated significantly in the adhesion tissues from3rd day, which were examined by immunohistochemical method. And the mesothelium covering the surgical sites and the adhesion tissues healed on7th day. Conclusion:The clamping trauma to the duodenum can result in significant postoperative intraperitoneal adhesions formation, which represents an ideal rat model for intraperitoneal adhesions research and prevention. And myofibroblasts may play an important role in the forming process of intraperitoneal adhesions.3. Experiment study of the prevention of QingreJiedu Herbs and LiangxueHuoxue Herbs on postoperative abdominal adhesion in ratsObjective:To observe the prevention of QingreJiedu Herbs and LiangxueHuoxue Herbs on postoperative abdominal adhesion in rats and explore the mechanism. Method:160Wistar rats were randomly divided into8groups and then made into postoperative abdominal adhesion model by duodenum clamped; protective measures or medicines were given respectively in the7days. Adhesions war evaluated on the14th post-surgical day, specimens were collected for histological staining and grading. Results:QingreJiedu Herbs, LiangxueHuoxue Herbs and their combinations can reduce the adhesion formation after the operation. QingreJiedu Herbs can inhibit the inflammation, the fibrosis and the neovasculrization of the surgical sites or adhesion tissues, and the formation and deposition of type Ⅰ, Ⅲ collagen were also depressed. Conclusion:QingreJiedu Herbs, LiangxueHuoxue Herbs, their combining and sequential applications can all depress the postoperative abdominal adhesion formation; the effect of QingreJiedu Herbs is the best. And the anti-adhesion mechanism may be related to their anti-inflammatory effects.4. An antibody microarray assay of postoperative abdominal infection in rats Objective:To screen specific markers of postoperative abdominal infection in rats using an antibody microarray. Method:20Wistar rats were randomly divided into experimental group and control group; peritoneal ravage fluid, serum, plasma and lymph fluid were collected3days after the operation, and tested using antibody chips. Results:19,20and22kinds of proteins were respectively elected from the peritoneal lavage fluid, serum and plasma. Lymphatic fluid failed to result. Among which, Integrity αMβ2, TRAIL singled out in3kinds of samples, and CINC-2α/β, FADD, MuSK, IL-3, IL-4, TIE-2, TLR4in2. Conclusion:The serum and peritoneal lavage fluid are ideal specimens for monitoring postoperative abdominal infection, the value of the9cytokines needs more supporting information. |
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