The Experimental Study Of MXG And C3aR Antagonist On Prevention Postoperative Abdominal Adhesion

Objectives Establish the mice abdominal wall-cecal injury adhesive model to evaluate the effect of mXG hydrogel with C3 aR antagonist on postoperative adhesion.Methods 48 mice models of abdominal wall-cecal injury adhesive were established and divided into 4 groups respectively.For the positive control group,0.5mL saline was injected into the abdominal wall and cecum and treated intravenously with 100?l PBS;for the C3 aRa control group,0.5mL saline was injected into the abdominal wall and cecum and treated intravenously with C3 aR antagonist(1mg/kg);for the mXG control group,0.5 mL 4% mXG hydrogel was injected into the abdominal wall and cecum and treated intravenously with 100?l PBS;for the mXG/C3 aRa group,0.5 mL 4% mXG hydrogel was injected into the abdominal wall and cecum and treated intravenously with C3 aR antagonist(1mg/kg).6 mice were killed in each group 7 and 14 d after operation to evaluate the degree of adhesion and the histological observation.36 mice models of abdominal wall-cecal injury adhesive were established and divided into 4 groups respectively.Grouping and intervention were the same as above.On Days 1,3 and 5 after operation,the wall-cecal injured tissues and serum of three mice in each group were collected for analysis of C3,C3 aR immunohistochemistry(IHC),mRNA expression of C3 aR,cytokines like TNF-?,IL-1?,IL-10,to explore the mechanism of anti-inflammatory function of C3 aR antagonist.Results 1 7d,14 d after the operation,a severe 4~5 score adhesion with fibrous connective tissue hyperplasia formed in positive control group.A 3~4 score adhesion with loose connective formed in C3 aRa control group.A 1~2 score adhesion formed in mXG group with some inflammatory cell infiltration.Whereas most cecum and abdominal wall began to heal in mXG/C3 aRa group with less connective tissue.2 Immunohistochemistry and Real time-PCR results: On Days 3 after operation,the positive expression of C3 was observed in the injured tissue in all groups,mainly in macrophage and vascular endothelial cells,positive expression weakened On Days 5.On Days after operation,the positive expression of C3 aR was observed in the injured tissue in all groups,Real time-PCR results showed that the mRNA level of C3 aR peaked at the site of injury,On Days 5 after operation,the mRNA level of C3 aR decreased gradually,and the positive staining decreased.3 Luminex results: The serum levels of TNF-? in the C3 aRa group and mXG/C3 aRa group were significantly lower than those in the mXG/Con group On Days 1,3 and 5 after operation(p<0.05).The serum levels of IL-1? in the C3 aRa group and mXG/C3 aRa group were significantly lower than those in the mXG group/Con group On Days 1,3 and 5 after operation(p<0.05).The serum levels of IL-10 in the C3 aRa and MXG/C3 aRa groups were significantly higher than those in the MXG/CON group On Days 1,3 and 5 after operation(p<0.05).4 C3 aR mRNA Real time-PCR: On Days 1,3 and 5 after operation,the expression of C3 aR mRNA was significantly increased in the four groups of internal reference,and the difference was no significant,on the third day each group reached their highest level.Conclusions 1 Gross,adhesion scores and histological results showed that the mXG thermosensitive hydrogel with C3 aR antagonist can effectively prevent postoperative abdominal adhesions.2 All the experimental groups showed positive expression of complement components C3 and C3 aR at the injury site,indicating that the complement system was activated during injury,inflammation,and adhesion.3 C3 aR antagonist can reduce TNF-?,IL-1? levels in serum by inhibiting excessive activation of complement and promoting the release of anti-inflammatory cytokine IL-10.m XG hydrogel have better effect on postoperative abdominal adhesion prevention with C3 aR antagonist.