5637 Cell Lines In Vitro And Establish Nude Mouse Animal Model

Background:Bladder cancer is the most common tumor of the urinary system. In clinical, bladder cancer is usually divided into superficial tumors confined to mucosa and myometrial invasive tumors. An important way to treat patients with myometrial invasive bladder cancer is systemic chemotherapy. However, the traditional chemotherapy regimens (GC, MVAC) show poor efficacy and serious toxicity. So, a new method, which is called as molecular targeted therapy, begins to be valued to improve these shortcomings. This method has higher selectivity, and lower toxicity. In order to evaluate the efficacy of the molecular targeted drugs, the best way is to establish an animal model of bladder cancer to be used in drug trials.Objective:Get information about the growth state of bladder cancer5637cells in vitro, and establish an animal model to prepare for the next drug trials.Method:Take the frozen bladder cancer5637cells out of liquid nitrogen, recover and subculture them. When the shape and growth rate of cells are good, we can start to draw the growth curve. Make the bladder cancer cells which are under logarithmic growth phase into cell suspension, and inject it into subcutaneous of2nude mice. After a few weeks, we take out the tumor and cut it into small pieces. Transplant these small pieces into subcutaneous of another4nude mice. Observe the growth state of tumors and measure their volume. At last, we take out the tumors to do pathological examination.Result:The growth rate of bladder cancer5637cells under subculture was good. We used time as the horizontal axis and absorbance as the vertical axis to draw the growth curve. Between the first four days, bladder cancer cells were under logarithmic growth phase. Four days later, they went Into the plateau phase.2-3weeks after the2nude mice had been injected with bladder cancer cell suspension, the tumors achieved the maximum volume. Similarly,2weeks after the4nude mice had been transplanted with tumor pieces, the tumors achieved the maximum volume.Conclusion:Heterotopic transplantation tumor model of bladder cancer in nude mice has high tumor formation rate and fast grow speed. The most influencing factor with the volume of tumors is experimental operation.