Effect Of 5-allyl-7-gen-difluoromethylenechrysin On The Growth Of Human Cervical Cancer Cell Line HeLa Cultured In Vitro

Objective: To investigate the effect of 5-Allyl-7-gen-difluoromethylenechrydsin (AFMC) on inhibition of growth in cervical Cancer Cells Cultured in Vitro, and its mechanism.Methods: Human cervical Cancer (HeLa) cells and human umbilical vein endothelial cells (HUVEC) cells were cultured in vitro. The trypan blue exclusion method was used to investigate the growth inhibitory effect of AFMC in HeLa cells and HUVEC cells; Colony-formation assay in plate and soft agar was used to test inhibition of colony formation of HeLa by AFMC. Flow cytometry(FCM) using PI staining was used to analyse the cell cycle change after treatment with AFMC. ERK1/2 and Cyclin D1 protein expression level of HeLa cells was detected by western blot.Results: AFMC inhibited the growth of HeLa cells in a dose and time-dependent manner, its efficacy was stronger than that of Chrysin, while there was no significant effect on HUVEC cells. AFMC also suppressed the anchoring-dependent and anchoring-independent growth of the HeLa cells in a dose-dependent manner. FCM analysis indicated that various concentration of AFMC can significantly arrest the cell cycle in G1 phase of HeLa cells. Western-blotting analysis indicated that after treatment HeLa cells with AFMC, the protein level of phosphorylated ERK1/2 and Cyclin D1 was down-regulated in a dose and time-dependent manner. Conclusion:1. AFMC significantly inhibited the growth of HeLa cells, in a dose-dependent manner, its efficacy was stronger than the Chrysin, while there was no effect on HUVEC cells.2. AFMC inhibited growth of HeLa cells is associated with it arrested the cell cycle in G1 phase.3. The inhibition of growth of HeLa cells by AFMC is associated with downregulation of phosphorylated ERK1/2 and Cyclin D1 protein level, as to the arresting cell cycle in G1 phase.