Study On The Expressions Of The HGF/c-met In The Vitro Cultured Normal Oral Fibroblast And Hacat Cell Line Stimulating By Different Concentration Arecaline

ObjectiveObserving the expression and change of hepatocyte growth factor (HGF) and hepatocyte growth factor receptor(c-met) in the vitro cultured normal oral fibroblast and Hacat cell line after stimulating by different concentration arecaline,to study the potential pathogenesis mechanism of them in oral submucous fibrosis(OSF).MethodsCultured normal oral fibroblast and Hacat cell line in vitro,then stimulating them by different concentration arecaline such as 0 ug/ml(control group) ,10 ug/ml (G10 group) , 20ug/ml (G20 group) , 40 ug/ml (G40 group ), 80 ug/ml (G80 group) .And then using the reverse transcriptased polymerase chain reaction(RT-PCR) to detect the expression of HGFmRNA and c-metmRNA in these groups.Results(1)In the vitro cultured oral fibrosis compared with the control group ,the 10,20,40,80ug/ml groups expression high HGF concentration (P < 0.05). And 40 , 80ug/ml groups expressed higher than 10, 20ug/ml groups ( P < 0.05), but there is no more obviously difference between 10and 20, 40and 80ug/ml (P > 0.05).(2)In the vitro cultured oral fibroblast compared with the control group, the c-met concentrations are all highly expressed,but here is no distinguished difference about c-met expression concentration between 10and 20, 40and 80ug/ml groups (P > 0.05).(3)In the vitro cultured Hacat cell line, compared with the control group ,the 10, 20, 40, 80ug/ml groups expressing high c-met concentration ( P < 0.05),and here is no distinguished difference between 10and 20, 40and 80ug/ml groups (P> 0.05).(4)In the vitro cultured Hacat cell line,there is no HGF expressing in all of the groups.(5)The HGF expressed by cultured oral fibroblast and the c-met expressed by Hacat positive correlation,the correlation coefficient is 0.965(P<0.05).Conclusion(1)Arecaline can enhance the vitro cultured oral fibrobiast express HGF, demonstrated that FB can reactor an anticytotoxicity, HGF/c-met maybe concentrate to the cell injury and repairing process.(2)Arecaline can enhance the vitro cultured Hacat cell line express c-met, but dosn't expressing HGF,indicated that arecaline maybe a c-met mutation promoter.(3)Arecaline stimulated the Fb expressed HGF and stimulated Hacat expressed ,there existed a positive correlation between them,suggested that HGF/c-met was a circulatory mediator between epithelium tissue and connective tissue.