The Study Of The Corelationship Between Spleen Deficiency Syndrome And Psoriasis Vulgaris

Objectives1. Study the relationship between spleen deficiency syndrome and psoriasis, to provide scientific theoretical basis for clinical treatment.2. Preliminary to explore psoriasis intestinal immune function, so as to improve the study of the pathogenesis of psoriasis.Methods1. Clinical Research:From the 317 patients with psoriasis vulgaris,52 patients met the spleen deficiency syndrome, analysising 52 cases of patients gender and age composition ratio, and the severity of psoriasis, PASI score and differentiation from blood case. At the same time,15 patients were screened from the 52 patients for the study, with 15 cases of psoriasis patients with non spleen deficiency and 15 healthy subjects for comparison. Patients took Stool specimens in the morning,-80℃ sealed. It reserved for the detection of intestinal flora diversity.2. Animal experiment:52 female C57BL/6 mice were randomly divided into four groups:psoriasis with spleen deficiency modeling group, psoriasis modeling group, spleen deficiency modeling group and control group. Psoriasis with spleen deficiency modeling group and spleen deficiency modeling group were orally given bitter and cold purgative Senna decoction combined with limitating of nutriention to make spleen model, for a continuous duration of 21 ds, and added psoriasis modeling process in the first 16 days of the spleen deficiency model ing.At the ending of 21 days the two models were completed. The mice were observed with activity, spiritual, urine, hair, and other general signs. And the psoriasis model ing mice was evaluated according to the psoriasis area and severity index (PASI) and pathological change. The expression of IL-17, IL-6, IL-23, TNF-α, TGF-β in intestinal tissue were detected by ELISA. The expression of Th17 and Treg cells of CD4+T lymphocytes in mice mesenteric lymph nodes were detected by flow cytometry.Results1. General information on psoriasis patients with spleen qi deficiency analysis:The 52 patients included male to female ratio was 1.2:1, the patients aged 40-50 constituted the main; PASI score of rash was low, the body parts of erythema, scaling, infiltration compared:the legs erythema, BSA, PASI compared with other parts were extremer. head scales were significantly higher than all other parts. And the infiltration of body parts had no significant difference. Combined with the results from the blood syndrome, most patients with see dryness, blood stasis.2. The diversity of the intestinal flora:compared three stool samples, the intestinal flora abundance index of psoriasis with spleen qi deficiency group were higher than the normal group; The diversity index and F/B values of psoriasis with spleen qi deficiency group and psoriasis without spleen Qi deficiency group were higher than the normal group, and psoriasis with spleen qi deficiency group compared with the normal group, the difference was significant.3. The results of animal experiments:3.1 Psoriasis spleen deficiency model:After modeling mice decreased body weight, decreased activity, curled up, get together, messy hair, stool abnormal spleen deficiency performance, the back of mice saw typical psoriasis-like rash of skin erythema, scaling, infiltration, and pathological picture is also in line with pathological changes of psoriasis, suggesting that psoriasis with spleen deficiency mice model success.3.2 Immunity index Results:the content of IL-17, IL-23, IL-6, TNF-α, TGF-β in the intestinal tissue and the expression of Th17 cells in mesenteric lymph nodes of psoriasis modeling group was significantly higher than the control group (P<0.05); Psoriasis with spleen deficiency modeling group, the spleen index and Th17 expression increased significantly compared with the control group (P<0.05); Spleen index of spleen deficiency modeling group is lower than the normal group, and the cytokine IL-23, TNF-a, TGF-β1 content is higher than the normal group (p<0.05).Conclusion1. According with the information of patient into the analysis, patients of psoriasis vulgaris with spleen qi deficiency may be more common in middle-aged, rash lower extremities as the most important.2. Patients of psoriasis vulgaris may exist intestinal flora imbalence, the merger may increaseed while with the spleen qi deficiency syndrome.3. The results of the intestinal flora showed that Firmicutes was the most important advantage bacteria to patients of psoriasis vulgaris with spleen qi deficiency syndrome, while closing to Desulfovibrionaceae and Desulfovibrionales of Proteobacteria.4. Model of psoriasis with spleen deficiency appeared late in psoriasis, may for the immunosuppression of spleen deficiency.5. model of psoriasis showing Increased intestinal inflammatory cytokines and Th17 cells, may be accompanied by intestinal immune function abnormalities. Model of psoriasis with spleen deficiency showing Increased Increased Th17 cells, suggesting that the model also exists intestinal immune function abnormalities.6. Mice modele of psoriasis with spleen deficiency showing spleen deficiency typical performance and psoriasis-like rash changes, prompted model of psoriasis with spleen deficiency for success.