The Experiment Of Jianpijiedu Decoction On Guinea Pig Model Of Psoriasis With Spleen Deficiency Syndrome

Objective:To establish the Guinea Pig Model of psoriasis with Sleen DeficiencySyndrome and study the function as well as dose-effect relationship of jianpijiedudecoction.Methods: Guinea Pig were divided into8groups by randomization, blank1groupand blank2group,experimental1group and experimental2group with10Guinea Pig in eachgroup; chinese herbs high dose,the medium-dosage group and low dose groups, controlgroup, with12Guinea Pig in in each group. Except blank1group and blank2group, theexperimental1group established the psoriasis model with5%propranolol emulsion, othergroups after successfully establishing the psoriasis model with5%propranolol emulsion,choose to give gavage of bitter and cold purgative rhubarb twice a day for a continuousduration of7d to injure the spleen and stomach in order to duPlicate a clinical like spleendeficiency model, then with ELISA test kit for the guinea pig serum TNF alPha IL-20content changes,conduct visual inspection and pathological section observation on ear skinlesions among guinea pigs of all groups.Result:(1)5%propranolol emulsion brought outthe psoriasis-like lesion,after established the model, experimental1group and blank1grouphas significant difference on baker grade (P<0.01);after give gavage of rhubarb,experimental1group and blank1group has significant difference on spleen deficiencysyndrome grade and weight(P<0.01);(2)jianpijiedu decoction has effect on guinea pig serumTNF alpha and IL-20content, after give gavage of jianpijiedu decoction and Acitretincapsule respectively, the serum TNF alpha and IL-20content of the high dose and themedium-dosage chinese herbs groups became lower compared with experimental2group(P<0.01),the varying concentrations of the herbal medicine showed the obviousdose-effect relations.(3)jianPijiedu decoction has effect on psoriasis-like histopathologicaland,after give gavage of jianpijiedu decoction and Acitretin capsule respectively, the lesionhistopathological were improved to some extent of the three chinese herbs and control groupand there has significant difference compared with experimental2group (P<0.01),the result showed the higer of the dose and the better of the effect,and the chinese herbs high dosegroup better than control group(P<0.05);(4) jianpijiedu decoction has improvement onSpleen Deficiency Syndrome,control group and experiment2group has no significantdifference on weight(P>0.05),three chinese herbs has significantdifference(P<0.05).Conclusion:(1) The psoriasis spleen deficiency syndrome grouppreliminarily has the pathological features of psoriasis, which are similar to TCM spleendeficiency syndrome symptoms.(2) jianpijiedu decoction has effect on psoriasis may relatedcontrol its inflammatory reaction and immune adjustment;(3)jianpijiedu decoction not onlyimprove the psoriasis-like lesion but also inmprove the spleen deficiency syndrome.