Professor Liu Qingsi On The Treatment Of Osteoporosis Academic Thought

Objective:First, the heritage and development of the thinking of Professor Liu Qingsi academic thinking on systems analysis, and summarized, followed by Professor Liu Qingsi on the Treatment of osteoporosis in theory to be organized and studied. Center of this division is intended to Professor Liu on behalf of academic thinking side of the GuKang study:1. Observed by animal experiments in ovariectomized rat serum active vitamin D and bone mineral density changes;2. Clinical observations of GuKang by side osteoporosis in postmenopausal osteoporosis patients and non-active subjects serum vitamin D and bone mineral density changes, and apply these indicators GuKang intervention study. To reveal:(1) enzyme activity, the strength of the kidney and the presence of osteoporosis within the necessary link;(2) whether the severity of the disease osteoporosis through the activity of1α-hydroxylase expression;(3) From the enzymatic point of view of "renal bone" meaning, enrichment and development of Chinese "kidneys bone" theory;(4) proof of Bushenzhuanggu deeper, spleen qi, promoting blood circulation network of the rule is GuKang and their representatives, parties of the effectiveness of osteoporosis prevention and treatment, scientific, practical and clinical significance.Method:Animal experiments:60healthy female SD rats,4months old, average weight of230±20g. Randomly divided into control group, model group, Lo cover the whole group, GuKang group, a total of four groups of15. Meet a week later, all rats with2%pentobarbital sodium40mg/kg intraperitoneal injection of anesthesia, and then open the abdominal cavity, removal of small pieces of fat control group, the rest of the group ovariectomized. In addition to the control group, all groups were exposed to90days with Cdcl2liquid. While the control group with normal saline, with Lo Lo cover the whole group at the same time cover the whole, while GuKang group fed with GuKang.24h collection of urine before death.2%of all rats with40mg/kg intraperitoneal injection of sodium pentobarbital anesthesia to collect blood samples. Take three parts of the calculation of bone mineral density, right femoral neck (BMD1), Ward District (BMD2) and2-4lumbar spine (BMD3). Serum estradiol E2,25(OH) D3and1,25(OH)2D3measured.Clinical study:select volunteers to participate in this study of60postmenopausal women, into Non-osteoporosis group and osteoporosis group, using dual-energy X-ray absorption spectrometry and radioimmunoassay measurement of bone mineral density and serum activity of two groups of vitamin D, the correlation analysis.Results:Animal experiments:1.End of the experiment a total of48rats into the final test statistics.2.The OVX group BMD of femoral neck and lumbar spine were significantly lower than the control group, the difference was significant (P<0.05); the ovariectomized group showed no significant difference between groups.3. Groups of ovariectomized estradiol levels were significantly lower than the control group (P<0.05), that the success of ovariectomy surgery.4.The serum25(OH)D3levels were not different. The group of ovariectomized rats, serum1,25(OH)2D3levels were significantly lower than the control group (P<0.05), showed that cadmium exposure can make1,25(OH)2D3levels drop, but does not affect25(OH)D3. GuKang in the blood1,25(OH)2D3levels increased, although no statistically significant difference, but there are still clinical significance.5. The exposure group blood and urine of cadmium values were higher, and there was significant difference (P<0.05). Meanwhile, the blood cadmium and urine cadmium were significantly correlated.6. Compared with the control group, ovariectomized rats increases urinary calcium excretion, urinary each exposure group, β2-MG and NAG levels, were higher. GuKang on renal function subjects had a significant effect, this effect is positive, positive.Clinical findings:1. Before treatment between the two groups25(OH)D3and1,25(OH)2D3, serum estradiol and lumbar bone mineral density were significantly different (P <0.05). This difference shows that postmenopausal osteoporotic group of patients with osteoporosis, non-osteoporotic group, non-osteoporosis in postmenopausal subjects.2.6months after medication, these indicators between the two groups no significant differences, indicating that GuKang can significantly increase hydroxylase activity,25(OH)D3and1,25(OH)2D3, serum estradiol and lumbar bone density has increased.3. Before and after their osteoporosis group, these indicators have significant differences. That can significantly improve GuKang in the blood25(OH)D3and1,25(OH)2D3, serum estradiol levels, prompting increased bone mineral density.Conclusion:Animal experimental results:1. GuKang can increase bone density, increased levels of estrogen in the blood.Bone mineral density in ovariectomized rats exposed to cadmium, serum estradiol levels were significantly decreased, suggesting that estrogen hydroxylase activity and significantly affected:decreased activity, decreased synthesis. GuKang can improve bone mineral density, serum estradiol levels. This is a kidney function and GuKang contain estrogen-like substances.2. Hydroxylase activity can improve GuKang and promote active vitamin D synthesis.The serum25(OH)D3levels were not different. The group of ovariectomized rats, serum1,25(OH)2D3levels were significantly lower than the control group. Cadmium exposure in ovariectomized rats show hydroxylase activity severely inhibited, GuKang, although not fundamentally change the1,25(OH)2D3levels decreased, but tended to increase the value point of view, which may be applied to GuKang bone cells and intestinal epithelial cells and contain estrogen-like substances, resulting in improved renal hydroxylase activity, and promote the active vitamin D synthesis, but the mechanism needs further study.3. GuKang can significantly improve renal function, kidney has a strong effect.GuKang for renal function in rats exposed to cadmium can not radically change, but the survival rate of rats, hair, activity and diet were significantly improved detection of targets have changed.Clinical findings:1. Osteoporosis patients serum25(OH) D3andl,25(OH)2D3, significantly decreased serum estradiol, bone density is also reduced.Postmenopausal osteoporosis in patients with serum25(OH)D3and1,25(OH)2D3, serum estradiol were significantly decreased, lumbar spine bone mineral density also decreased significantly, suggesting that post-menopausal osteoporosis occurs, the internal mechanism of change, triggered osteoporosi s.2. Hydroxylase activity can improve GuKang and promote active vitamin D synthesis.GuKang, while not fundamentally reverse the serum1,25(OH)2D3levels decreased, but can improve the hydroxylase activity, and promote active vitamin D synthesis.3. Strong GuKang kidney function, a more comprehensive synergy.GuKang can improve the patients overall body function, can significantly reduce back pain, bone density decline to slow down, hydroxylase activity increased, active vitamin D synthesis increased.4. GuKang through the kidney, spleen, blood and thus can improve the hydroxylase activity, slow down the process of osteoporosis.5. Possible mechanisms are:GuKang through the kidney, strengthen the kidney hydroxylase activity, and promote the activity of renal vitamin D synthesis, involved in the regulation of calcium and phosphorus metabolism, delaying the process of osteoporosis; GuKang through health spleen, and promote intestinal and renal epithelial cells outside the synthesis of active vitamin D, to promote intestinal calcium absorption, bone cell activity, slow down the process of development of osteoporosis; GuKang through blood circulation, promote liver blood, spleen blood, accelerate blood circulation, promote hepatic synthesis of25(OH)D3to promote hydroxylase activity, which will help synthetic activity of vitamin D, osteoporosis slow process of evolution. Strong kidney, spleen health, blood smooth, hydroxylase activity, the slow osteoporosis, among them there is an inherent and inevitable contact. Enzymatic point of view of "renal bone" meaning, enrichment and development of the Chinese "Kidneys bone" theory.6. Deeper proof Bushenzhuanggu, spleen Qi, activating blood and GuKang network of representatives of parties the effectiveness of osteoporosis prevention and treatment, scientific, practical and clinical significance.