Effects Of Benefiting-bone Capsule On Levels Of DBH And ADRB2 In Bone Tissue Of Model Rats With Postmenopausal Osteoporosis

Objective:The research was to observe changes of Benefiting-bone capsule(BBC),a pure Chinese medicine compound preparation,on expression and distribution of dopamine-β-hydroxylase (DBH) andβ2-adrenergic receptor(ADRB2) in cells of bone tissue surface of model rats with postmenopausal osteoporosis(PMOP),explore some possible esoteric mechanism of connection among bone tissue,sympathetic nerve and its neurotransmitters,and preliminary study on targets and the possible mechanisms of Chinese medicine compound preparation treating PMOP.Methods:1.60 10-month-old Sprague-Dawley(SD) female rats were divided uniformly into model group and sham group.There are 48 rats in model group and 12 rats in sham group.Rats in the model group were duplicated with the method of breeding for 12 weeks after bilateral ovariectomy.The bone mineral density(BMD) of these rats was checked by Dual energy X-ray absorptiometry(DEXA) to assure that the experimental animal model of osteoporosis was replicated successfully.2.12 weeks later the rats with model replicated successfully in the model group were divided uniformly into untreated model group,low dose BBC group,middle dose BBC group and high dose BBC group.The rats in the low dose BBC group,middle dose BBC group and high dose BBC group were respectively treated with gastric perfusion of BBC solution that amounted to 1 time,3 times and 9 times dose of BBC used in clinic adult patients,and those in the sham-operated group and untreated model group received gastric perfusion of equal saline, once a day for 12 weeks.3.Then DBH and ADRB2 in cells on bone trabecula surface of distal femur of rats in every group were checked with immunohistochemistry method.4.Statistic software SPSS 13.0 was used and rank sum test was adopted for comparison of rank data among groups. Results:1.12 weeks after operation the BMD of many bone positions of rats in model group was significantly lower than that of rats in sham-operated group(P＜0.05).2.24 weeks after operation the expression levels of ADRB2 on the membrane of the first layer of cells in rat distal femur bone marrow cavity near the surface of bone trabecula of untreated model group were significantly higher than that of sham-operated group(P＜0.05); that of low dose BBC group,middle dose BBC group and high dose BBC group were significantly lower than that of untreated model group(P＜0.05);there were not significant difference between middle dose BBC group and high dose BBC group(P＞0.05).3.24 weeks after operation the expression levels of DBH in the cytoplasm of the first layer of cells in rat distal femur bone marrow cavity near the surface of bone trabecula of untreated model group were significantly higher than that of sham-operated group(P＜0.05);other treated groups were not significantly different from that of untreated model group(P＞0.05).Conclusions:1.10-month-old SD female rats had been made successfully into experimental animal model of PMOP by breeding for 12 weeks after bilateral ovariectomy.2.The increasing excitability of sympathetic nerve and expression of DBH and ADRB2 in cells on bone tissue surface and activing signal pathway of RANKL/RANK/NF-?B in bone tissue may be involved in the abnormality of bone rebuilding and contribute to the morbidity of PMOP.3.Dose-dependent declining excitability of sympathetic nerve and expression of ADRB2 in cells on bone tissue surface and suppressing signal pathway of RANKL/RANK/NF-?B in bone tissue may be the mechanism of BBC treating PMOP.