The Clinical And Experimental Research On The Treatment Of Anxiety With AnShenfang

While advances in science and technology have brought huge amounts of benefits to modern societies, they have also caused numerous side effects that increasingly threaten people’s heath. The modern world is fast-paced and filled with fierce competition at a global level, and as a result, millions of people are under more pressure than ever before, and most would have to face deteriorated working conditions such as longer working hours, the requirement of higher level of concentration, insufficient physical exercises, etc.. World Health Organization (WHO) reported that nowadays people pay more and more attention to mental health and psychological welbeing [REF]. It also pointed out that among the10diseases that are most costly to taxpayers,4are classified as mental illness. In addition, the treatment of mental diseases costs a quarter of the total budget on health. These facts have shown that mental health has brought an increasing amount of attention, not only from medical practitioners, but also governments and their citizens.Anxiety disorder is one of the biggest type of mental illness and it shows an increasing incidence in recent years. Anxiety disorder covers several different forms of abnormal and pathological fear and anxiety. It is divided into Generalized Anxiety Disorder (GAD) and Panic Disorder (PD), where GAD is a common chronic disorder characterized by long-lasting anxiety that is not focused on any one object or situation. Those suffering from GAD are frequently accompanied by physiological symptoms such as dry mouth, upset stomach, nausea,diarrhea, dyspnea, palpitations, frequent urination, impotentia,menstrual disorder, sweating, flushed face,①muscle tension and spasms.headache, short attention span, hypomnesis and sleep disorder. In China, along with the economic development in recent years, people have realized the importance of mental health. As people become more active in seeking medical advice on symptoms of anxiety disorder, hospitals and specialized clinics have admitted an increasing number of patients with such disease, which has driven a surge in research in the physiology of anxiety disorder and its treatment.Currently, modern medicine not only has made great progress in the pathogenesis of anxiety disorders, but also has a sound theoretical in drug treatment. There are two classes of drugs being mostly used in clinical treatment, benzodiazepine drugs such as Diazepam and non-benzodiazepine drugs such as Buspirone. The former ones effect quickly in the beginning, but often accompanied by lethargy, constipation, muscle relaxants, memory weakened, and other side effects. The latter ones, with fewer side effects, effect slower. Moreover due to the easy formation of dependence and tolerance, and withdrawal reactions, to some extent limits the application of this classes of drugs. Therefore to find out the drugs with efficacy and fewer side effects, has become an important topic of the current anti-anxiety research areas.After accessing large amounts of literatures and leaning from anxiety disorders researches of modern medicine, our subject of study finds out correlations between anxiety disorders and these disorders of Chinese medicine. Under the guidance of the doctoral mentor, the renowned Chinese Medicine Professor Chen Baotian, on the basis of summarizing,we establish three treatment principles, and set up AnShenfang combined with almost50years of clinical experiences of professor Chen, we use chaihu,guizhi,longgu,muli and others medicines in AnShenfang for the treatment of anxiety. This study observes the clinical efficacy of AnShenfang in treatment of Generalized Anxiety Disorder (GAD), and further with animal experiments, investigates the pharmacodynamics, morphology and the mechanism of actions of AnShenfang. Aimed to give full play to the concepts of Chinese Medicine and the advantages of treatment based on syndrome differentiation,combined with modern medical experiments, our study is designed to better explained the role of effects and mechanisms of AnShenfang, in order to explore and pave out an effective way for better treatment of anxiety disorders.chapter1. Observation of AnShenFang in Treating Generalized Anxiety Disorderl.Objective:To investigate the efficacy of AnShenFang(ASF) for patients with general anxiety disorder (GAD). To objectively estimate the therapical effect of "AnShenfang" in treating GAD through the clinical overall curative effect as well as the finding.2.Methods:Clinical curative effect observation:120cases according with the diagnostic standard were equally divided into (ASF) treatment group and western medicine treatment(DZP) control group at random.During treatment:The patients were entered a4-week randomized contralled triad.60patients of control group were given DZP and other60of treatment group were given ASF.other sedative and anxiolytic medicines should be abstinent.Mental nursing is necessary besides the therapy of during treatmentThe HAMA were measured before and after3,6,12months of the treatment. The results were compared with ASF treatment group and western medicine treatment control group.Assess the benefits and adverse reaction of ASF and DZP in the treatment of GAD, according to the level of Hamilton Anxiety Scale (HAMA) and Treatment Emergent Symptom Scale (TESS)Before and after treatment, temperature, heart rate, blood pressure, renal and hepatic funtion, plasmal electrolyte and electrocardiogram were assessed and compared.3. Results:After4weeks of treatment the HAMA decrease values in two groups were less than those before the treatment (P<0.01). There was on significant difference in response rate between the2groups. There was significant difference in TESS between the2groups.4.Conclusion:ASF is one of the effective therapies with less emergent symptom in treating GAD, which is worth studying and spreading further more.chapter2. The pharmacodynamics research on the treatment of anxiolytic with ASF1.Objective:using elevated plus-maze as a measure of anxiety in the rat to observe anxiolytic effect of ASF particles and do preliminary study of its mechanism.2.Method:Sixty male wistar rats were randomly divided into6groups (10rats/group):normal control group, model group, diazepam group, and high,medium and low dose ASF group; then used chronic emotional stress method to establish anxiety rat model and gave the drug intervention at the same time.40g.Kg-1for the high dose group.20g.Kg-1for the middle dose group,and10g.Kg-1for the low dose group and the saline for the control group;meanwhile,made the insomnia model for15day.Then evaluate their behavior by adopting internationally recognized elevated plus-maze test (EPM)3.Anxiety rats model preparation:using chronic emotional stress method to establish anxiety rat model by elevated plus-maze.4. Used statistical software SPSS13.0to detail the data.During the multi-groups compares uses the single factor variance analysis (One-Way ANOVA) to examine; All statistical analysis has the significance boundary as P<0.05.5. Result:Compared with normal control group, percentage rate and frequency rate of model group into open arm time was significantly reduced, and that of rats from diazepam group and ASF dose high, medium and low dose group was significantly raised comparing with model group, the most obvious raise group was ASF medium dose group.6. Conclusion:The pharmacodynamics research on the treatment of anxiolytic with ASF shoud that ASF provides anti-anxiety effect by elevated plus-maze(EPM) as a measure of anxietyChapter3. The laboratory explore on the treatment of anxiolytic with Anshenfang1.Objective:To observe anxiolytic effect of ASF particles and do preliminary study of its mechanism.2.Method:①. Sixty male wistar rats were randomly divided into6groups (10rats/group):normal control group, model group, diazepam group, and high,medium and low dose ASF group;②.Using chronic emotional stress method to establish anxiety rat model and gave the drug intervention at the same time to evaluate their behavior by adopting internationally recognized elevated plus-maze test (EPM)③.After behavioral observation, anesthesias executed rats of normal control group, model group, ASF medium dose group; separated rats hippocampus on the ice stage.④.Separated clarified liquid to determine the content of glutamic (GLU) and y-aminobutyric acid (GABA) by using western blot method;⑤.Evaluated GABAA receptor, N-methyl-D-aspartate receptor1expression through immunohistochemistry.⑥. Detect the receptor gene by the fluorescence quantitative polymerase chain reaction(FQ-PCR).3.Used statistical software SPSS13.0to detail the data.During the multi-groups compares uses the single factor variance analysis (One-Way ANOVA) to examine; All statistical analysis has the significance boundary as P<0.05.4.ResuIt:Compared with normal control group, model of glutamate in hippocampal tissue expression increased significantly compared with the normal control group and γ-aminobutyric acid expression decreased obviously; compared with the model group, glutamate acid expression in diazepam and medium ASF dose group was significantly low and y-aminobutyric acid expression was increased. While, there was no significant difference when comparing ASF medium dose group with diazepam group; compared with control group, GABAA receptor immune positive cells expression in model group was lower and amount of Immune positive cells expression of N-methyl-D-aspartate receptor1was increased; compared diazepam dosage group and ASF medium dose group with model group, the N-methyl-D-aspartate receptor1expression of the former were significantly reduced and GABAA receptor expression level were increased; while there was no significant difference in ASF medium dose group and diazepam group.5.Conclusion:ASF provides anti-anxiety effect,①.increase the content of y-aminobutyric acid in the brain, strengthen GABAA receptor and the receptor gene expression,reduce glutamic acid content,and decrease N-methyl-D-aspartate receptor1and the receptor gene expression. The best one is ASF medium dose group.