| Backgroundsince the ancient times,the humanity on has had the strong interest to the sleep and the dream.The humanity has not stopped regarding the sleep and the dream inquisition since Floyd's "Dream Analysis.As an important constituent,sleep play a important role not only in maintaining human's physiology healthy,but also psychological is.Normal sleep maintains the abundant energy physical strength to the human body,significance of sleep in relation to maintenance nervous system,the endocrine system,and the immune system function normal.In recent years,anxious and depression from accelerated society transformand and people's life rhythm,made the insomnia occur frequently.Insomnia is a kind of sleep barrier synthesis that something wrong in the onset and maintenance of sleep, far from satisfying the individual physiological needs,affecting the patient's life(for example the daytime sleepy asthenia,remembrance breakdown and so on).The modern medicine discovered the occurrence and the maintenance of sleep had a correlated with not only the suprachiasmatic nucleus,hypothalamus,ascending reticular activating system and apophysis cerebri,but also acetylcholin(ACH), noradrenalin(NE),dihydroxyphenyl ethylamine(DA),the single-amines neurotransmitt e,the amino acid neurotransmitte as well as melatonin,prostaglandin,interleukin.At present,the main drug to the insomnia is still BZD sedative hypnotics,which bring adverse reaction and drug dependence as well as treat insomnia.The differentiation of Traditional Chinese medicine,adjusts the human performance condition,achieves the improvement of sleep,has brought the gospel for the insomnia patient.Famous physician Chen Baotian of traditional Chinese medicine created "AnShenfang" through reading the Chinese medicine classics "Shanghanlun",and confirmed curatively for a long time in clinical work.simultaneously improved the patient's anxious and despondent.on the base of clinical observation foundation,we designs this experiment to observate the the therapeutic effect of the AnShenfang systematically,and further discusses the mechanism of AnShenfang through the animal experimentation,provides the reliable theory and the clinical basis for the development of AnShenfang.chapter 1.AnShenfang treats the insomnia clinical observationObjective To objectively estimate the therapical effect of "AnShenfang" in treating insomnia of Liver-qi and Changes into Liver-fire through the clinical overall curative effect as well as the Polysomnogram(PSG).Methods1.Clinical curative effect observation:80 cases are selected and randomly devided into two groups:the AnShenfang group and the control group;2.According to level of Chinese medicine symptom graduation foundation, compared improvement of main symptom(sleep time,difficulty falling asleep,easily awakening,dreaminess,fatigue),useing the non-parametric test;3.According to grades around the treatment,calculate "the curative effect index", evaluates rank of improvement in each patient,appraises the total curative effect;4.Evaluating the therapical effect though Polysonmogram(PSG),records each data(total sleep time,sleep latency time,awareness times,shallow sleep time,deep sleep time,REM sleep time).Objectively appraisal curative effect.Rusults1.Clinical observation:there was no significant differences in gender,age and course of disease in two groups(P>0.05),the two groups are comparable;2.Symptom curative effect appraises:there was no significant differences in "sleep time(Z=-0.633,P=0.527),difficulty falling asleep(Z=-1.716,P=0.086), dreaminess(Z=-1.115,P=0.265)" between two groups;there was significant difference in "easily awakening(Z=-2.285,P=0.022),fatigue(Z=-2.856,P= 0.004) "between two groups;3.The total curative effect appraises:there was significant difference in total curative effect(Z=-2.130,P=0.033),the treatment group total curative effect surpasses the control group;4.The polosomnography(PSG) curative effect appraisal:Before treatment,there was no significant difference in total sleep time compares(t=-0.153,P=0.879);sleep latency time compares(t=-0.400,P=0.691); awareness times compares(t=-0.733,P=0.467);shallow sleep time compares (t=0.290,P=0.773);the deep sleep time compares(t=-1.021,P=0.312);the REM sleep time compares(t=-0.425,P=0.673)between two groups.Two groups are comparable;Around treatment,comparison in treatment group:there were significant difference in the total sleep time compares(t=-3.534,P=0.002),awareness times compare(t=2.753,P=0.011),the deep sleep time compares(t=-4.853,P=0.000), REM sleep time compares(t=-4.596,P=0.000),there was no significance difference Regarding the sleep latency time compares(t=1.572,P=0.129),and the shallow sleep time compares(t=0.409,P=0.686).Ttreatment group has effect on"increasing the total sleep time,the deep sleep time,the REM sleep time,reduces awareness times;Symptoms comparison in control group:there were significant differences in the total sleep time compares(t=-2.757,P=0.011),the sleep latency time compares (t=2.324,P=0.029),the shallow sleep time compares(t=-2.396,P=0.025);there was no significant differences in awaken times compares(t=1.821,P=0.081),deep sleep time compares(t=-1.846,P=0.077),REM sleep time compares(t=-1.028,P=0.314), Control group has effect on"the increase sleep total time,the reduce sleep latency time,increases the shallow sleep time";After the treatment,there were significant differences in the shallow sleep time compares(F=9.042,P=0.004),the deep sleep time compares(F=4.887,P=0.032),the REM sleep time compare(F=8.441,P=0.006) between two groups.there was no in the total sleep time compare(F=0.394,P=0.533),the sleep latency time compare (F=1.633,P=0.208),awareness times compare(F=1.053,P=0.310),increaseing the deep sleep time,the REM sleep time curative effect in treatment group surpasses the control group;The control group increasing the shallow sleep time curative effect surpass the treatment group.Conclusion1.Curative effect observation:the effect of "easily awakening,fatigue" in the treatment group surpasses the control group's,and the overall curative effect of treat group surpass the control group;2.Polysomnogram(PSG) observation:The AnShenfang has significant effect on "the total sleep time,awareness times,the deep sleep time,the REM sleep time", there were significant differences in the "deep sleep time,the REM sleep time" Compared with the control group.The treat group mainly increased "total sleep time"through the increasing of"the deep sleep time" and "the REM sleep time";3.The treatment security,each inspection target does not have exceptionally.Chapter 2 laboratory explore of "AnShenfang" treat insomnia1.The Effect of "AnShenFang"on Central Neurotransmitters of insomnic RatsObjective Observe the effect of "AnShenFang"on Central Neurotransmitters of insomnic Rats,discusses the mechanism to treat the insomnia.Methods1.The experimental animal and group:60 Wistar rats,the body weight (200±20g),south the hospital experimental animal center provides,permit number: (Guangdong) 2006-0015.were randomly divided into the high dose group,middle dose group,lowdose group and the saline control group;2.Administration:40g.Kg-1 for the high dose group.20g.Kg-1 for the middle dose group,and 10g.Kg-1 for the low dose group and the saline for the control group;meanwhile,made the insomnia model for 15 day;3.insomnia rats model preparation:making insomnia rats model through limiting rats'movement:rats were Put in self-maderats movement limit meter,controls the rat's active space through the motion film threading,applies the brake 1 time each day, first time 4h,increases 1h each time after that,sustaining for 15d,diet and water were prohibited in this period;4.examination of the central nervous:separate both hippocampus and hypothalamus of the rat.weighting,glass homogenizer refining;centrifugalization, quantitating through the high performance liquid chromatogram(HPLC);5.Uses statistical software SPSS13.0 to detail the data.During the multi-groups compares uses the single factor variance analysis(One-Way ANOVA) to examine;All statistical analysis has the significance boundary as P<0.05.Rusults1.Gamma-amino-butyric acid(GABA) content in hippocampus:there were significant differences among the high dose group and saline control group compare (P=0.002),the middle dose group and saline control group compare(P=0.017),the low dose group and saline control group compare(P=0.045);2.Glutanic acid(GLU) content in hippocampus:there were significant differences among the high dose group and the saline control group compare (P=0.011),the middle dose group and the saline control group compare(P=0.045); there was no significant difference between the low dose group and saline control group compare(P=0.566);3.Gamma-amino-butyric acid(GABA) content in hypothalamus:there were significant differences among the high dose group and saline control group compare (P=0.001),the middle dose group and the saline control group compare (P=0.028);there was no significant difference between the low dose group and the saline control group compare(P=0.166);4.Glutanic acid(GLU) content in hypothalamus:there was significant difference between the high dose group and the saline control group compare (P=0.015);there were no significant differences between the middle dose group and the saline control group compare(P=0.103),as well as the low dose group and the saline control group compare(P=0.798).Conclusion The AnShenfang can elevate the Gamma-amino-butyric acid (GABA) content,reduces the glutanic acid(GLU) content both hippocampus and hypothalamus of the rats.2.The Effect of "AnShenfang"on serum cortisol of Sleep Deprivated RatsObjective To observe the effect of "AnShenfang" on Serum cortisol of Sleep Deprivated Rats.Methods1.The experimental animal and group:30 rats were randomized divided into 2 groups:treat group and normal control group,each group had 15 animals.Treat group were fed by "AnShenFang"and normal control group were fed by normal saline for 3 days,then sleep deprivated for 72 hours,test the body weight and content of serum cortisol on sleep deprivation 0h,24h,48h and 72h;2.Administration "AnShenfang"20g.Kg-1 for the treat group,and the saline for the control group;3.The rats sleep deprives model preparation:use"the multi-platform sleep deprivation methord" prepare sleep deprivation model(sleep deprivation,SD).The room temperature maintenancing 20-25℃,replacing the the water used every day, maintenancing free feed condition;4.Testing methord:sleep depriving for 72h Continuesly,records each group of animal body weight,and detects blood serum cortisol on 0h,24h,48h and 72h.Blood serum cortisol determination method:on7 AM draws blood 0.5ml from the tail vein, centrifuge at 3000r/min for 10min,preserve serumin the low temperature refrigerator for detect(-60℃);5.Uses statistical software SPSS13.0 to detail the data.use the analysis of covariance(ANCOVA)around test;uee the ANOVA for the repeated measures and Independent-Samples T Test to test the data on the different time All statistical analysis has the significance boundary as P<0.05.Rusults1.There were significant differences in the body weight of the two groups compare(F=79.682,P=0.000);2.There was no significant difference on body weight at the 1,2nd day between the treat group and the control group's compares(1st day:t=-0.429,P=0.671;2nd days:t=1.835,P=7.077),but there were significant differences on bodu weight between weo group on the 3,4th day(3rd days:t=3.675,P=0.001;4th days:t=5.675, P=0.00).there was significant difference on body weight on different time(F=460.01, P=0.000).Between the grouping and the time has the correlation(F=70.22,P=0.000);3.There were significant differences in the serum cortisol of the two groups compare(F=70.601,P=0.000);4.There was no significant difference on content of serum cortisol at the 1,2nd day between the treat group and the control group's compares(1st day: t=-0.372,P=0.712;2nd days:t=-0.699,P=0.490),but there were significant differences on content of serum cortisol between two groups on the 3,4th day(3rd days:t=3.675, P=0.001;4th days:t=5.675,P=0.00).there was significant difference on content of serum cortisol on different time(F=460.01,P=0.000).Between the grouping and the time has the correlation(F=70.22,P=0.000).Conclusion1.The speed of body weight of Treat group was slow compared with saline control group;2.The speed of content of serum cortisol of treat group was slower than compared with saline control group;3.Anshenfang granules can elivate the rat's anxious symptom which reduce the sleep deprive.
|
PDF Full Text Request