Clinicopathological Study About Malignat Tumors In Canine And Feline Based On The WHO Classification

The incidence rate of malignant tumor in felines and canines showed increased frequency. In recent years, the classififelineion of the tumor of the WHO is also in place to adjust. In order to understand the occurrence of malignant tumor in animals, the factors and the principles of classififelineion, Establishment of an animal malignant tumor database as early as possible. At the same time provide a reference for the diagnosis of human tumors. In this study, canines and felines as experimental animals. According to the latest WHO classififelineion criteria for malignant tumors will be studied in canines, felines, case specific classififelineion. In cases of canines and felines by H. E. staining, immunohistochemistry, Igh and TCR gene rearrangements, PCR method were differential diagnosis. At the same time, canines and felines were compared, as well as with the comparison of human tumors. Through clinical and pathological changes of malignant tumor in canines and felines diagnosis. Based on the summary of the clinical characteristics of the new WHO classififelineion of malignant tumor in canines and felines.The specific contents are as follows:(1) Make a nationwide collection of canines, felines, malignant tumors of the case for epidemiological investigation. According to the different tissues of malignant tumor, the classififelineion and statistics of malignant tumor of the feline were carried out, and the etiology of breast cancer and malignant lymphoma in the high incidence of malignant tumor were analyzed.First, in the course of the investigation, the organization of the malignant tumor of the canines and felines was determined and recorded. In this process, if there are different types of malignant tumors in the same organization to take their growth is more dominant, the source of the higher degree of deterioration of the tumor is the final diagnosis of records. Effects of age and gender,breed and inheritance on the occurrence of tumor. Statistical methods will be applied to statistical analysis of the final diagnosis of malignant tumors and draw a chart to analyze.(2) At the same time, the malignant tumor tissues of canines were classified, and the immunohistochemical expression of each malignant tumor was recorded, and then the immunohistochemical characteristics of canines and felines were analyzed.(3) In this study, canine, feline cancer cases as the material, according to the new version of the WHO classififelineion of tumors of a detailed diagnosis. In such cases, we will identify malignant tumors in canines and felines are detailed classififelineion to WHO specificfelineegories. Collection of cases covering more general animal tumors. In the case of canines and felines have been found in relatively rare cases.(4) In malignant tumors, lymphoma cases are the focus of the identififelineion of the object,using a variety of methods to identify the diagnosis. First according to the clinical manifestations of the initial judgment. After the production of tissue sections, Applifelineion of H.E. staining and immunohistochemical methods for the diagnosis. In canine, feline malignant lymphoma cases were detected by the methods of gene rearrangement and PCR amplififelineion in addition to immunohistochemistry and HE staining. Gene rearrangement based on the surface of lymphocytes with antibodies or antigen specific binding capacity of such a class with a number of glycoproteins.These glycoproteins are composed of subunits. These include B lymphocytes produced by IgH and IgL and T cells produced by TCR. The gene encoding of both is similar, generally by the variable region(V region), variable region(D region), connecting region(J region) and constant region(C region). The malignant tumor is a clonal proliferation in the early stage of hematopoietic cell differentiation in the lymphatic system. The rearrangement of the immunoglobulin(Ig) gene and T cell receptor(TC) gene is the most reliable marker. So we can use the method of gene rearrangement to detect.In the summary, the results of this study are as follows: The incidence of skin and soft tissue malignant tumor in canines is the first place of malignant tumor in canines, followed by breast cancer. Felines have a higher proportion of lymphoma. Followed by skin and soft tissue tumors and breast cancer.Canine, feline malignant lymphoma is a malignant tumor with high incidence in lymphoid hematopoietic tissue, and its difference in sex, age and so on. Malignant histiocytoma,malignant meningiomas respectively for canines, felines occurred in mesenchymal tissue, nerve tissue has high incidence of malignant tumors. In the aspect of immunohistochemistry, multiple expression of keratin in epithelial tissues, over expression of vimentin mesenchymal tissue etc.Malignant lymphoma in the B cell lymphoma of the immunohistochemical diagnosis of B markers positive, such as CD20, CD79 a and other.CD10 positive Bcl-2 follicular lymphoma positive. T cell lymphoma was diagnosed with T markers positive, such as CD3 and so on. Other tissue tumors can be seen as a specific expression of the molecule, also found in the tumor to identify the differential diagnosis of immune marker molecules. In the process of comparing canines and felines with malignant tumors, it is found that the immunohistochemical and pathological changes are similar to those of the human.