| Perianal tumors are tumors that develop on or close to the anus, and it accounts for 80% of all tumors that occur in the perianal area. The correlative research of tumor typing and Immunohistochemical detection that has been reported less. Therefore, deep studies on perianal tumors has very great meaning to guide clinical practice. The primary objective of this study is to investigate and research the clinical characteristic, histologic features, cellular and molecular biologic changes, and to provide a basis for early diagnosis.In the studies,58 cases that suffered from perianal tumors we collected were monitored and studied, investigated including such aspects as tendency of ages, gender, medical history, feeding condition, coat color, location, and size of tumor. The dates of CBC and biochemistry of affected patients were investigated and analyzied in detail. The tissues of 38 attacked cases and 6 controlled cases were obtained by using paraffin sectioning and H.E stain to evaluate the cell morphology and to diagnosis cancer type; Also all specimens including 38 samples and 6 controled cases were examined using immunohistochemistry to check estrogen receptors (AR, ER, PR), CK, P53 and CK5. And analysis dates regarding in nuclei of interphase cells by image processing in 400 times measuing the average ratio of nucleus to cytoplasm, the average nuclear area, the average cell circumference and the average round degree, totally 169 views and 18883 cells.Median age is 12.6yrs in the 58 cases, and all are male. The most risk were Beijing dogs, pomeranians, cocker spaniels, this result is not consistent with other reports. It may be due to the popularity of a certain breed of dog. The brown and yellow coat dogs were worst affected, with a higher rate of incidence than other coated dogs. There was not related with biochemistry dates in these cases. The survival rate of surgical removal of the tumors was higher in non-surgical treatment, and it was seen that castration can reduce the recurrence of perianal tumors. Benign tumors were 23 cases, 60.5% in affected cases by paraffin sections in pathology assessment. There were 14 malignant perianal gland adenocarcinoma, approximately 36.8 percent of the total. Besides that, there was 1 perianal gland epithelial tumors that is quite rare. The rate of Benign (23)/malignant (14) was 1.64:1. By comparison, the studies in immunohistochemistry and histopathology found both R expression in control groups and sample groups. ER and PR were positive and P53 were negative in all the perianal tumors, but detection of cytokeratin CK(AE1/AE3) found 37 positive samples,97.3% of total. CK5 and CK(AE1/AE3)in normal perianal tissues of control groups were negative and positive in perianal tumors tissue. The results showed that nuclear area (MNA) had a trendency to increased by nuclei of interphase cells by image processing。MNA were 1:1.23 in normal controlled groups, moderately differentiated adenoma and adenocarcinoma were 2.06:1 and 2.38:1 respectively, These had significant differences as compared with that of normal subjects (P<0.01).According to the results from the experiment, cytokeratin changes may be the important difference between benign and malignant tumors. The detection of CK5 has primary significance of differential diagnosis, can assist early diagnosis and typing for perianal tumors. The average nucleoplasm ratio in perianal tumors in dogs is proportional to the tumor differentiation, the average cell nucleus roundness is inversely proportional to that. A detailed observation and description for different types of perianal tumors in histopathology will provide a basis and method for typing, diagnosis and treatment. |
