Research On Genetic Structure Of Anemone Amurensis (korsh.) Kom.、Iris Laevigata Fisch. And Phylogeographic Relationship Of Genus Anemone And Iris

Genetic structure means the spatial and temporal patterns of genetic variations,or unrandom ditributions of genetic variations in different populations.Polyploidy plays an important role in the evolution of plant genomes. In thepresent study, in order to investigate the polyploidy events within the genus Anemone,we developed eighteen microsatellite markers from the hexaploid species A.amurensis and tested their transferability in five closely related species. According tothe results, the number of total and polymorphic alleles for each locus varied from oneto eight and zero to eight, respectively. The polymorphism information content (PIC)for these microsatellites ranged from0.00to0.71. In addition, most of thesemicrosatellites can amplify successfully in the congeneric species. Thesemicrosatellites primers provide us an opportunity to study the polyploid evolution inthe genus Anemone.In this study, genomic and expressed sequence tag (EST)-derived microsatellitesare developed and characterized from Iris laevigata and also tested theirtransferability in I. ensata, I. setosa, I. halophila, I. scariosa, I. potaninii, I. tenuifolia,I. bloudowii and I. sanguinea.10genomic and6EST-derived microsatellites werecharacterized in I. laevigata. These microsatellite primers amplified one to5alleles inthe species I. laevigata and some of these primers were also successfully amplified incongeneric species. These microsatellite primers provide us an initial set of molecularmarkers to explore the spatial population genetic structure of I. laevigata. In addition,these markers may also be useful in populations and conservation genetic studies ofclosely related species.Phylogenetic relationships of Iridaceae species inferred from trnL-F and rps16sequences data had been studied. The trnL-F and rps16genes were analyzed andcompared to construct the phylogenetic trees using Clustal x、PAUP and DNAMANsoftware. The phylogenetic analysis implied that Belamcanda chinensis was asingleton branch, far away from other irises, so it hould be treated as an indepndentgenus—genus Belamcanda. Iris dichotoma (Pardanthopsis dichotoma) was closelyrelated to other irises, it should be kept in genus Iris, instead of being treated as anindepent genus: Pardanthopsis. The species of Genus Iris in this study were separatedinto two parts．Determining relationship of Iridaceae species through the study ofchloroplast DNA, this study aims to provide a basis for breeding and improvement.A new forma: Iris cathayensis Migo f. alba M. Z. Sun et H. X. Xiao et D. W.Zhou is described and illustrated. The new forma belongs to Sect. Limniris and isclosely related to I. cathayensis, but differs in the color of the flowers: of this formaare white, while flowers of I. cathayensis are violet-blue.