| ObjectiveThe steroid-induced necrosis of the femoral head was high in the whole world.One of the results of necrosis of the femoral head is osteoarthritis if it isn't treat effectively. The necrosis of the femoral head was reversible through appropriate treatment in the early.This experiment studied the effect of the Traditional Chinese Medicine called Chuangu tablets on the expression of BMP-2,FGF,VEGF,Bcl-2, VP,TGin the bony tissue of necrosis of the femoral head and serum of the rabbits which were induced by subcutaneous injectin of methylprednisolone sodium succinate.To investigate the effects of Traditional Chinese Medicine Chuangu tablets on the experimental necrosis of the femoral head induced by methylprednisolone sodium succinate in the rabbits.Further to evaluate the prosthetic effects of Chuangu tablets on the bony tissue of necrosis of the femoral head, remodel the femoral head,and explore its possible bone remodeling molecular mechhanisma on both Signal Transduction Pathway.MethodsNecrosis of the femoral head was induced in rabitts by subcutaneous injectin of methylprednisolone sodium succinate.Sixty rabitts were randomly divided into 5 group; normal control group(N group,12 rabitts), model control group(M group,12 rabitts),positive control group(P group,12 rabitts),high dose of Chuangu Tablet(H group,12 rabitts), low dose of Chuangu Tablet(L group,12 rabitts),The rabbits of M,P,H and L group were disposed with injection of solumedrol injection 20mg/kg,once 3 days,at the time H and L groups were treated with Chuangu tablets,once Iday,and Pgroup was treated with Zhuogui pills,once 1 day.42 days later,all the rabbits were sacrificed.The pathological change of the femoral head were observed by HE staining under microscope.The protein expression of VEGF and BMP-2 were determinged by immunohistochemical staining method.Serum levels of VP and TG were dectected..Results:General status:At the end of 42 days normal control group was unchanged.The fur color pattern inmodel control group got parched unkempt,the activity and cibation reduced. The status between the high dose of Chuangu tablets group and positive control group were better than that of model control group.HE stain for the bony tissue:In the model control group, Empty lacunaes increased Liposome was found in the cytoplasm and nuclei of the osteocyte in early period.Pyknosis,karyolysis and karyorrhexis were observed in the osteocyte..There was obviously lipid droplet.The structure of plasma menbrans was not complete, obvious screvice could be found in collagenous fiber.But, in the high dose of Chuangu tablets group and positive control group, Empty lacunaes increasing wan in slight extent. Pyknosis,karyolysis and karyorrhexis were not obviously observed in the osteocyte.Immunohistochemisty stain for the bony tissue:Buffy represents positive result.. The normal control group is the most obvious by the positive mass of BMP-2,FGF and VEGF.The positive mass of BMP-2,FGF and VEGF increased gradually in the order of model control group, low dose of Chuangu Tablet group, positive control group(P group,high dose of Chuangu Tablet, normal control group.But, the positive mass of Bcl-2 decreased gradually in the order of model control group, low dose of Chuangu Tablet group, positive control group(P group,high dose of Chuangu Tablet, normal control group.The data from immunohistochemistry are puantitied by Image-Pro Plus 6.0 analysis program. BMP-2,FGF and VEGF of the model control group, low dose of Chuangu Tablet group, positive control group,high dose of Chuangu Tablet is significantly different from the normal control group (P<0.05).And those masses of the low dose of Chuangu Tablet group, positive control group,high dose of Chuangu Tablet is significantly different from the model control group (P<0.05). those masses of positive control group,high dose of Chuangu Tablet is significantly different from low dose of Chuangu Tablet group.Regarding of the high dose of Chuangu Tablet and positive control group,there is not significantly different between (P>0.05).VP and TG:Compared with the model control group,,serumVPA and TG (P< 0.05) were significantly different from low dose of Chuangu Tablet group, positive control group,high dose of Chuangu Tablet, normal control group. Serum TG and VP of low dose of Chuangu Tablet group, positive control group,high dose of Chuangu Tablet is significantly different from the normal control group (P<0.05). Serum TG and VP of low dose of Chuangu Tablet group is significantly different from positive control group,high dose of Chuangu Tablet (P< 0.05). Regarding of the high dose of Chuangu Tablet and positive control group,there is not significantly different between (P>0.05).ConclusionThis experiment researches the effects of Traditional Chinses Medicine Chuangu Tablets on the prevention and cure of steroid-induced necrosis of the femoral head in rabitts.The study reveals:Traditional Chinses Medicine Chuangu Tablets not only have a protective effect on steroid-induced necrosis of the femoral head and can definitely inhibit development byt necrosis of bony tissue,but also promote the prosthetic effects of Chuangu tablets on the bony tissue of necrosis of the femoral head, remodeling the femoral head.The possible remodeling the femoral head molecular mechanisms of the effects of Chuangu Tablets decoction may due to up-regulating expression of BMP-2,bFGF,VEGF,Bcl-2; up-regulating Expression of BMP-2,bFGF Signal Transduction Pathway can promote osteoblasts multiplication and differentiation;VEGF Signal Transduction Pathway can promote differentiation of epithelial cells in micrangium of the femoral head; Bcl-2 Signal Transduction Pathway is anti-apoptosis of bony cell. Moreover, Chuangu Tablets can regulate fat metabolism and rheology of the blood. Chuangu tablets can promote the expression level of VEGF and BMP-2 of callus tissue in steroid-induced necrosis of the femoral head, This may be one of the mechanisms of Chuangu tablets to prevention and cure of steroid-induced necrosis of the femoral head. protein...
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