The Effect Of Ultrashort About The Rabbit With Steroid-induced Avasular Necrosis Of Femoral Head On Vegf Mrna And Bmp-2 Mrna

Objective:To observe the variation of VEGF and BMP-2 as well as patho-change in the steroid-induced avasular necrosis of femoral head under the effect of ultrashort wave,Discussing the control efficiency and mechanism of ultrashort wave on SANFH, to provid the theoretical basis for clinical application. Methods:The experiment use New-Zealand healthy big rabbit for study and research, and adopt horse serum combined with hormone to build a model of steroid-induced avasular necrosis of femoral head.The test is divided in two stages, first stage:to build the model, there are 26 rabbits in the model group,4 in the normal group. After modeling, the model group is divided into ultrashort wav group and control group randomly. This is the second stage.The ultrashort wav group was gived ultrashort wave after estallishing model.There are two treatment courses,each one is fifteen days and an interval of five days,the first one is non-heating ultrashort waves therapia,the second one is eupyrexia.therapia, no therapia to the control group. Taking X-ray examination every stage and getting femoral head for specimen fixation,decalcification. patho-exam to confirm the model done well, Employing real time fluorescent quantitation PCR to detect the variation of VEGF,BMP-2. Results:â‘ Horse serum combined with hormone can estallish a model of steroid-induced avasular necrosis of femoral head,in the modelled group, dot and flake osteoporosis, density inhomogeneity, blurred bone trabecula on the femoral head through X ray,From the pathological section, the empty osteocyte lacunae more, bone marrow cells instead of fat, bone nucleus pyknosis.â‘¡Histopathology observation:the dellen rate of vacuity bone cell of the ultrashort wave group is smaller than the control group (p<0.05); there are much more angiogenesis, lots of osteoblast in the he ultrashort wave group and there are too many fat cells and flake empty osteocyte lacunae in thel.8018.â‘¢Real time fluorescent quantitation PCR:At the end of the first treatment course, the expressions of VEGFmRNA of the ultrashort wave group was 1.8018 times that of the control group, At the end of the second treatment course, the expressions of VEGFmRNA of the ultrashort wave group was 3.8238 times that of the control group, p<0.01, The results have statistical significance; At the end of the first treatment course, the expressions of BMP-2mRNA of the ultrashort wave group was 2.7438 times that of the control group, At the end of the second treatment course, the expressions of VEGFmRNA of the ultrashort wave group was 10.1260 times that of the control group, p<0.01, The results have statistical significance; Conclusion:1,Ultrashort wave can improve avascular necrosis of the femoral head microcirculation, Reverse patho-process of avascular necrosis of the femoral head,2,Ultrashort wave can increase the expressions of BMP-2 and VEGFmRNA, and improve it's own reactivation ability, thus amendment the proceeding of avascular necrosis of the femoral head.