Experimental Studies On Effects Of Electroacupuncture On The Nuchal Region On The Expression Of HIF-1α And It's Relative Target Genes Following Cerebral Ischemia Reperfusion In Rats

As a familiar disease,Apoplexy has taken place frequently and the ratioof death and lameness are both very high.With the application ofthrombolytic therapy,the reperfusion injury is paid to more and moreattention.How to reduce cerebral ischemia-reperfusion injury and enhanceneuronal tolerance to ischemia and hypoxia,has gradually become a researchhotspot.The elctro-acupuncture on nuchal region(EANR)n the acute stageof cerebral ischemia and effects of the treatment period achieved significantefficacy,but efficacy of the mechanism of the needle is just beginning.Theissue of needle-intervention from the power of hypoxia-inducible factor(HIF-1α)and related gene expression in the target to start for theacupuncture treatment of ischemic encephalopathy study provides a newmechanism for the research.Objective:To observe the expression of HIF-1αand it's correlative geneof in ischemic penumbra in rats after acute cerebral infarction and to explorethe effect and the possible mechanism of electro-nape acupuncture therapy.Methods:The model of focal cerebral ischemia was made by blockingthe middle cerebral artery with monofilament in rats.The middle cerebralartery ocelussion rats were randomly divided into 4 groups:sham group(group A),model group(group B),electro-acupuncture group(group C)and electro-nape acupuncture group(group D)Male Wistar rats weredivided randomly into four groups:sham group,model group,electro-napeacupuncture group and electro-acupuncturegroup;each group was separatedinto 12h,1d,3d,5d after rerperfusion as subgroup for investigation.HEstaining and electron Electron microscope were used to look into the changesof histomorphology.Took Immunohisto chemistry and In situ hybridizationto detect the expression of HIF-1α(mRNA),EPO(mRNA),GLUT-1(mRNA),and VEGF observe ultrastructure in brain tissue,nerve functionscore. Results:Electro-nape acupuncture has the obvious auxoaction onrecuperation of neurologic impairmentThe results showed electro-napeacupuncture possessed protective effect against cerebral ischemia-reperfusion injury,compared with the model group.The results indicatedthat compared with group B,the neuron ultrastructure was mended in groupC and in group D(P＜0.05,P＜0.01)It was more dominant in group D thanthat in group C(P＜0.05)Electro-nape acupuncture can lessen neurologicimpairment.Results pointed out,that compared with the model group,electro-acupuncture group and electro-nape acupuncture can increase theexpression of HIF-1α(mRNA),EPO(mRNA),GLUT-1(mRNA)andVEGF.Conclusion:1.Electro-nape acupuncture treatment to save the cerebral ischemicpenumbra of nerve cells around the infarction to enhance blood supply tobrain tissue,to improve nerve function.2.Electro-nape acupuncture can promote ischemic penumbra glial cellsand capillary hyperplasia,reduce neuronal damage and improve theultrastructure of nerve cells.3.Electro-nape acupuncture of ischemic penumbra of the ultrastructure ofnerve cells improved synaptic membrane activezone increased significantly,to increase synaptic vesicles.4.Electro-nape acupuncture treatment by increasing HIF-1α,EPO,GLUT-1,VEGF protein expression to the nutritional nerves,vascularremodeling and restoration of the role of energy supply,thereby enhancingthe nerve cells of hypoxic-ischemic tolerance,reducing cerebralischemia-reperfusion injury.5.Electro-nape acupuncture treatment by increasing HIF-1αmRNA,EPOmRNA,GLUT-1mRNA expression to the nutritional nerves,vascularremodeling and restoration of the role of energy supply,thereby enhancingthe nerve cells of hypoxic-ischemic tolerance,reducing cerebral ischemia-reperfusion injury.