Study On Effect And Mechanism Of Injection "Xiao-Bi-Ling (XBL)" On Degenerative Osteoarthritis

Degenerative Osteoarthritis (OA) is the one of the four elderlypatients in China. It effects seriously on work and live ofPeople. Along with our country population aging, OA prevalence ratereaches as high as the total population 8.3%.Should sickness bedraws back by the articular cartilage becomes the core, Implicatesthe ossein, And inctudes outside the synovial membrane ligamentumcapoulare and the ligamentum capoulare the other structure varyingdegree inflammatory affection. Has involved the cell level and themolecular level to its research.[Objective] Study on Xiao-Bi-Ling injection on the treatment of OA.[Methods] Health adult Japan big ear white rabbit 50.0A modelanimals were randomly divided into 5 groups:group A, group B,group C, group D and group E. Animals in group A received XiaoBiLinginjection and animals in group B received Sodium hyaluronate (HA),groupC was sterilized normal saline intramuscularly, groupD wascontrol and received nothing and another group(groupE)was used fornomal control. Each group of 10.Under the same level conditionraises one week later, Besides E group, The other groups are improvedmethods of production model Hulth experimental of OA. With 3%pentobarbitol sodium 30mg/kg after rabbit ear reason intravenousanesthesia, lies supine on the bundle the rabbit and the surgerytable, takes the left knee inside vertical margin, long about 4cm,investigation ticulare not primary pathological change, cut-offinside ligamentum accessorium and first overlapping ligament,complete excision inside meniscus, the cascade sutures the wound,the sterilized dressing and the bandage wrap are fixed. After thetechnique, the anti-SARS treats for 3 days, minute cage raising, every day forces the animal to move 30 minutes. After making themodel one week later starts injects the medicine, A group injectsXiao-Bi-Ling injection 0.3ml, B group injects the Sodiumhyaluronate 0.3ml, C group injects physiological saline 0.3ml,once a week, gives the medicine 8 weeks continuously. D group ofanimal does not give the medicine, the simulation apprehends.Research target: After treatment 8 weeks later, each group takes8 rabbits stochastically, Examination the joint synovial fluid IL-1and TNF-αcontent, Simultaneously carries on the specimenobservation and under the synovial membrane cartilage's lightmicroscope the pathological change, Immunohistochemical detectionof cartilage MMP-1, TIMP-1 expression, as well as the use of PCRto detect MMP-1, TIMP-1 expression of mRNA.[Results] Xiao-Bi-ling treated group Injection osteoarthritisanimal histology and hemorrheology significantly reduce thepathological changes, obviously the cartilage destroys reduces,Synovitis disease inhibit, The Mankin'S grading has the distinctimprovemen(P＜0.01); knee joint synovial fluid, IL-1 and TNF-αconcentration decreased (P＜0.05), Articular cartilage in the MMP-1expression was significantly reduced, TIMP-1 expression wassignificantly enhanced, Electron microscope, synovial A, B cellsreturn to normal functional status.[Conclusion] Xiao-Bi-ling Injection intra-articular injection cansuppress the disease Synovitis, Reduces in the joint harmful cellfactor Quantity, alleviates to cartilage's destruction.