The Effect And Mechanism Of ZiGanBuShen On The Osteoporosis Model Rats Using Ovariectomize Method

Objectives: This research subject is plans to treats the ovariectomized rats with the traditional Chinese medicine compound prescription and the ethylene female phenol ,and be observed ovary osteoporosis model big mouse blood serum sex hormone level FSH, LH, E₂, insulin type growth factor - 1 (IGF-1) the level change, the internal organs index determination, the bone tissue pathology change, the RT-PCR method observe IGF-1, the OPG-mRNA expression in bone tissues to discuss the traditional Chinese medicine compound prescription to go to the ovary ossein sparse model big mouse's therapeutic action and to affect the mechanism.Methods:1. Blood sex hormone target determination: Picks the eyeball to draw blood, the separation blood serum, emits the immunization to determine the blood serum sex hormone : estradiol (E₂), the graafian follicle stimulin (FSH), the yellow body production hormone (LH)2. Blood bone metabolism biochemistry target determination: Picks the eyeball to draw blood, the separation blood serum, emits the immunization to determine the blood serum bone calcium element (BGP), insulin type growth factor - 1 (IGF-1).3. Womb wet weight, internal organs index, bone tissue pathology change: Beheads the execution the animal, drifts away the womb organization, calculates the internal organs index; Right flank takes out the thighbone, is fixed by the formaldehyde, takes the femoral head/stock backbone place to make the pathological section, makes a paraffin wax package of block, HE dyes, under the optical microscope carries on the pathology observation.4. Bone specimen RT-PCR: Left side beheading executes the big mouse, takes out the thighbone completely, puts in the liquid nitrogen to freeze, and loads separately in the aseptic EP tube seals, prepares examines IGF-1, the OPG-mRNA level.Results1. After been ovariectomized, the big mouse be formed the experimental osteoporosis; the blood serum E₂ level reduces obviously, FSH, the LH level elevates obviously; Blood serum BGP, the IGF-1 level obviously reduces; The womb wet weight and the internal organs index reduce obviously; The bone cerebral cortex thins, the bone density and the area reduce; IGF-1, the OPG-mRNA expression level obviously is lower than normally, presented the model osteoporosis mouse.2. The nutritious hepatorenal traditional Chinese medicine may adjust the reproduction endocrine system to a certain extent, reduces FSH, the LH level, and elevates the E₂ level.3. The traditional Chinese medicine compound prescription can obviously elevate the blood serum BGP, the IGF-1 level in model mouse.4. The traditional Chinese medicine compound prescription can elevates the big mouse's womb wet weight and the internal organs index; and can reduces the big mouse's osteoporosis degree.5. The traditional Chinese medicine compound prescription can elevates the IGF-1-mRNA, the OPG-mRNA expression state in bone tissue.Conclusion;The possible mechanism of Bushen Huatan Treatment ofosteoporosis is; Huatan Treatment to inhibit adipogenic differentiation of BMSCs, and Bushen Huatan Treat- ment to promote osteoblastic differentiation of BMSCs.