Experimental Study On The The Effect Of OPGmRNA And RANKLmRNA And Effect Of The Tectology Of Bone About The Method Of Bu Shen Jian Pi On The Osteoporosis Rat

Object : To study the effect of OPGmRNA and RANKLmRNA and the effect of the tectology of bone and about the method of Bu Shen Jian Pi on the Osteoporosis rats.Methods : Thirty six rats aged 10 months were randomly divided into three groups:the Bu Shen Jian Pi(BSJP) group,the viod group and the model group .The BSJP and model groups were performed ovariectomy on both sides,the void group rats were shamly operated. After one week begin to give the medicines,one tim in a day.Then kill the rats 12weeks latter. Pick out the 3th lumbar vertebra and store it in the liquid nitrogen.Rat mRNA in the bone were extracted by Trizol ,as followed the expression of OPGmRNA and RANKLmRNA were obtained with RT-PCR.On the other hands,take the outlying 1/3 quarters of femoral near the joint to investigate the tectology of bone.Result : The expression of BSJP group rats'OPGmRNA and RANKLmRNA compared with the modele group rats'(P<0.01),the result of OPGmRNA/RANKLmRNA of BSJP group rats compared with other two groups(P<0.01). On the other hands, the change of BSJP group rats'static tectology of bone compared with the modele group rats'(P<0.01),the result of dynamic tectology of bone of BSJP group rats compared with the model group (P<0.01).Conclusion : The method of Bu Shen Jian Pi can inhibit RANKL expression clearly , deep the bone remodeling, and inhibit the high switch rate of bone after menopause.