Research On Compatibility Rule And Pharmacodynamics Of Shou Tai Wan

Objective:Pharmacological experiments,the effect of Shou Tai Wan(STW) on abortion animal models of corepus luteum inhibition due to renal deficiency,were performed in order to bring out the optimal extration method,the optimal proportion and the compatibility rule of STW.Methods:Experiment 1:Collection of Genuine Medicinal Materials and Pharmacognostic identification of Semen Cuscutae,Herba Taxilli,Radix Dipsaci,Colla Corii asini were prepared in accordance with Chinese Pharmacopoeia.SA rats model of corepus luteum inhibition due to renal deficiency was made by intragsstric administration of hydroxycarbamide 450mg/kg/D in dioestrus.Mifepristone(RU 486) was used by intragsstric administration in 10^th day.SPF 70 pregnant SD rats,10 weeks old,weight 280～300g were randomly divided into 7 groups:â‘ normal control group;â‘¡model group(Hydroxyurea + mifepristone);â‘¢positive control group(Hydroxyurea + mifepristone + dydrogeterone);â‘£STW A(aqueous extract solution of STW);⑤STW B(ethanol-extracts of STW);â‘¥STW C(aqueous extract solution of guffs after alcohol extracting);⑦STW B+C(ethanol-extracts of STW + aqueous extract solution of guffs after alcohol extracting). Generalized case of rats in every group was observered.Rats were killed after 24 hours,the following data was measured:embryos number,abortion rats sand diameter of embryos.Decidua tissue on one side was collected in 30 minutes after rats death in asepsis condition to detect expression of PR by quantitative RT-PCR.Serum estradiol(E₂),progestogen(P) were measured by chemiluminescence.Experiment 2:8 combinations of STW were obtained by uniform design.SPF 110 pregnant SD rats,10 weeks old,weight 280～300g were randomly divided into 11 groups:â‘ normal control group;â‘¡model group(Hydroxyurea + mifepristone);â‘¢positive control group(Hydroxyurea + mifepristone + dydrogeterone);â‘£8 combinations of STW by uniform design.Animal model building and detection Methods were similar to Experiment 1.The optimal proportion of STW in theory was obtained by multiple regression analysis,canonical correlation analysis,optimum analysis,integrated weighting analysis.Experiment 3:SPF 60 pregnant SD rats,10 weeks old,weight280～300g were randomly divided into 6groups:â‘ model group(Hydroxyurea + mifepristone);â‘¡positive control group(Hydroxyurea + mifepristone + dydrogeterone);â‘¢clinical routine dose of STW;â‘£STW1(Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=15: 21:21:0);⑤STW 2(Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=20: 10:10:0);â‘¥STW 2(Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=30: 21:21:5).Animal model building and detection Methods were similar to Experiment 1.Results:Experiment 1:1.Intake of food/water,changes of pregnant rat body weight in 4^th and 9^th day,organ index in pregnant rats:compared with model group,there was no significant difference in all groups(P＞0.05).2.Abortion rate of pregnant rat:compared with model group,there was significant difference on abortion rate between model group and other groups (P＜0.05).Compared with positive control group,there was no significant difference in STW A group,STW B group,STW C group and STW B+C group(P＞0.05).3.The mean levels of serum E₂:compared with model group,there was no significant difference in STW A group,STW B group,STW C group and STW B+C group(P＞0.05).4.The mean levels of serum P:compared with model group,there was no significant difference in STW A group,STW B group and STW C group(P＞0.05). The mean levels of serum P in STW B+C group was significantly different from model group(P＜0.05).Compared with positive control group,there was no significant difference in STW A group,STW B group,STW C group,STW B+C group (P＞0.05).5.Expression of PR:compared with model group,there was no significant difference in STW A group,STW C group(P＞0.05).Expression of PR in STW B group and STW B+C group was significantly different from model group (P＜0.05).There was no significant difference between STW A group and STW C group(P＞0.05).6.The mean relative percentages compared with model group,which was calculated by Weighted Average Method,indicated that STW B+C group was better than other groups on SA rats model model7.Multivariate analysis of variance on 4 indexes of pharmacodynamic experiment indicated that STW B+C group＞STW B group＞STW C group＞STW A group in abortion rate of pregnant rat;STW B+C group＞STW A group＞STW C group＞STW B group in the mean levels of serum E₂;STW B+C group＞STW A group＞STW C group＞STW B group in the mean levels of serum P;STW B+C group＞STW B grou＞STW A group＞STW C group in expression of PR.It is suggested STW B+C group was better than other groups on SA rats model model.Experiment 2:1.Intake of food/water,changes of pregnant rat body weight in 4^th day:compared with model group,there was no significant difference in all groups(P＞0.05).2.Organ index in pregnant rats:there was significant difference between control group and model group(P＜0.05).Organ index of pregnant rats in positive control group,group2,group4,group5,group6 was significantly different from model group(P＜0.05).3.Abortion rate of pregnant rat:there was significant difference on abortion rate between control group and model group(P＜0.05).Abortion rate of pregnant rat in positive control group,group1,group2,group4, group5,group6,group7,group8 was significantly different from model group (P＜0.01).4.The mean levels of serum E₂:there was significant difference on the levels of serum E₂ between control group and model group(P＜0.05). The mean levels of serum E₂ in positive control group,group1,group2,group3 was significantly different from model group(P＜0.05).5.The mean levels of serum P:there was significant difference on the levels of serum P between control group and model group(P＜0.05). The mean levels of serum P in positive control group,group1,group2, group3,group4,group6 was significantly different from model group(P＜0.05).6.Expression of PR:there was significant difference on expression of PR between control group and model group(P＜0.05).Expression of PR in group1,group2,group3,group4,group5,group6 was significantly different from model group(P＜0.05).7.The Compatibility Rule of STW:7.1 Qualitative Analysis of contribution degrees of single medicine in STW:Semen Cuscutae＞Herba Taxilli=Radix Dipsaci＞Colla Corii7.2 Qualitative Analysis of interactions of single medicine in STW:It is suggested that abortion rate of pregnant rat is associated with interactions between Semen Cuscutae and Herba Taxilli,interactions between Semen Cuscutae and Colla Corii.The mean levels of serum E₂ is related to interactions between Semen Cuscutae and Herba Taxilli,interactions between Herba Taxilli and Radix Dipsaci.The mean levels of serum P is associated with interactions between Semen Cuscutae and Herba Taxilli, interactions between Herba Taxilli and Colla Corii,interactions between Radix Dipsaci and Colla Corii.Expression of PR is associated with 125interactions between Semen Cuscutae and Herba Taxilli,interactions between Semen Cuscutae and Colla Corii,interactions between Herba Taxilli and Radix Dipsaci.8.Amount of each crude drug in 8 combinations of STW and the mean levels of serum E₂ and P,expression of PR results of 8 combinations of STW on abortion rat models are interrelated by quadratic regression. The equation(R²=0.9832,P＜0.05) (?)=35.0123-0.2204*X1*X3-0.02321*X1*X4++0.2165X2X3+0.01078*X1² was obtained. The optimal proportion of STW are(Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=15:21:21:0).9 It was concluded from authentication experiment that compared with model group,there was significant difference in STW 1 group(Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=15:21:21:0),STW 2 group(Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=20:10:10:0),STW 3 group (Semen Cuscutae:Herba Taxilli:adix Dipsaci:Colla Corii=20:10:10:5).Conclusion:1.Abortion animal models of corepus luteum inhibition due to renal deficiency,which was established by Professor Luo Song Ping,successfully analogue renal deficiency of spontaneous abortion.