Researches Of Lymphocyte Function And Its Potassium Channels(Kv1.3) Induced By Exercise-Stress

Object:To explore the interaction of kinesio-stress,lymphocyte and body,We hope establish systematize model that puts together cell function and structure to try to kwon cell.Through researching K+ passage in immunity cell,inquirying kinesio-stress how to influence the immune system,the basic cause of exercise triggering immunity function.Method:In our experiment,we reproduce exhausted sport stress,to appalied lymphocyte transformation experiment,molecular biology, pharmacology and RVD.Result:After experiment,this study arrived at following results: After acute exhausted sport and exhausted sport for two weeks,(1) Spleen T lymphocyte descends to the ConA sensitivity,the function is repressed; (2)RT-PCR and Western blot show the different stress sets of periphery blood,splenic and thymic the mRNA of T lymphocyte of the Kv1.3 and its expression are obviously repressed.Howerve,those of the mRNA of the B lymphocyte Kv1.3 and the expression have no obvious difference with matched control set;(3)In the hypotension environment,exhausted sport sets of RVD regulatory ability descend obviously,especially exhausted sport for two weeks set;(4)The flow cytometry cell cycle analyzes: Compared with matched control set,acute exhausted sport and exhausted sport for two weeks each period cell the number all take place a change, the G0/G1 period cell percentage has increment slightly,but have no statistics difference(P＞0.05),the S period cell significantly decrease, the percentage of the G2/M period cell increase,Apoptosic rate increase(P＜0.05);(5)Exhausted sport sets make the CD4+ cell percent in thespleen T lymphocyte increment(P＞0.05),the CD8+ cell percent also has decreasetrend but hasn't statistics difference comparison with matched control(P＞0.05).Avalue of CD4+/CD8+ are all in the normal scope,but value of CD4+/CD8+＞2.0 in set of exhausted sport for two weeks.(6)This experiment also confirmed that dexamethasonecan suppress RVD of periphery blood T lymphocyte of normal small mice in vitro and have a quantity effect relation.Dexamethasonecan suppress RVD of periphery blood T lymphocyte of small mice of acute exhausted sport set in vitro and have a quantity effect relation.But different density dexamethasonecan(10-3 mol/L,10-5mol/L,10-7mol/L) can suppress NVD of periphery blood T lymphocyte of small mice of exhausted sport for two weeks set in vitro and haven' t a quantity effect relation:Conchsion:(1)Two kinds of exhausted sport can influence immune system,through lowering T cell activity,but few influence to the B lymphocyte function:(2)Sport stress regulates the immunity function through the ion passage,which explains the potassium passage participates cell-mediated immunity suppression;(3)The exhausted sport should effect the RVD function of potassium ion passage:(4)The exhausted sport sets of cell proliferation may be got difficulty in S period;(5) The exhausted sport for two weeks should arouse cell immunologic derangement:(6)GC influenced potassium passage of the peripheral blood T cell of mouse of exhausted sport for two weeks,which makes the K+ in the cell outflow decrease,then repress RVD;...