Experimental Study Of Systemic Lupus Erythematosus T Cell Clones

Object:Through researching the change of variable region of SLE patients' T cell receptorβchain(TCRVβ),to search T cell clone which related with disease syndrome.Method:Collected 12 cases of SLE patients,classified on the base of Phlegm and Blood stasis,set up normal contrast group and RA control group.Each case was draw-off 5ml of peripheral blood,deployed RT-PCR technique,amplified the district of TCRVβCDR3.Through silver staining of denaturing polyacrylamide gel electrophoresis to observe the spectral pattern picture of TCRVβand analyzed the characteristic of T cell clone's hyperplasy;carded out twice PCR on the hyperplastic abnormal strap,sequenced,compared and analyzed.Result:1.25 V gene family of TCRβin health adult PB had expression,the powerfulness and weakness of strap presented Gaussian distribution.2.Compared SLE patient with health people,the abnormal change of TCRVβfamily clan increased obviously,it had statistical significance(P=0.013＜0.05);compared RA patient with health people,although abnormal family clan had invariably increase, it had no statistical significance.3.The amount of abnormal TCRVβfamily clan in Phlegm and Blood stasis pattern of SLE patient had obviously increase compared health people(P=0.000＜0.01),it had statistical significance compared non-Phlegm and Blood stasis pattern of SLE patient(P=0.009＜0.01);non-Phlegm and Blood stasis pattern of SLE patient's abnormal family clan had increase compared health people,it had no statistical significance,but it had significant statistical difference compared Phlegm and Blood stasis pattern.Complex pattern increased obviously than single pattern.4.Number SLE5,SLE7 and RA1 patients' Vβ25CDR.3 districts were completely coincident(CASSLRGSTEAFF).The three kinds of patients differentiated Blood stasis;number SLE1 and RA1 patients' Vβ12CDR3 districts were completely coincident(CAISELREGDMSSGNTIYF);SLE1 was Phlegm complex Blood stasis,RA1 was Blood stasis.Number SLE12 and RA3 patients' Vβ4CDR3 districts were completely coincident(CSVGGLAGETQYF), SLE12 was Phlegm complex Blood stasis,RA3 was Blood stasis.Number SLE9 patient was coincident with abnormal amplification of T cell CDR3 series related GVHD and CMV of HBSC PG(CASSPGDEQFF),it belong to VB11 family, SLE9 was non-Phlegm and non-Blood stasis paracmasis patient.Conclusion:1.TCRVβpedigree can use as one of micro indexes to evaluate Phlegm and Blood stagnated SLE.2.Different autoallergic disease had identical series,it inferred LREGDMS,LRGS,GGLAG and PGD were motifs related with autoallergic disease antigen.3.Pattern had its material foundation,the same pattern might exist identical series,it personalized on molecular level.CASSLRGSTEAFF, CAISELREGDMSSGNTIYF and CSVGGLAGETQYF might have invariably dependability with Blood stasis pattern.Motifs LREGDMS,LRGS,GGLAG might the T cells those dependent with Blood stasis pattern in autoallergic disease.