Allergy Theory And Its Peripheral Blood Gene Expression Profiles

Objective:Analysis the ancient and modern recognition by Sorting pertinent literature,Supplement and consummate the theory system of the Allergic constitution;Study the peripheral blood gene expression profile of the Allergic constitution by gene chip technique.Not only providing theory and experiment support for identifying the Allergic constitution but also for the prevention and treatment of hypersensitivity disease.Methods:Analysis the ancient and modern recognition by Sorting pertinent literature,Discuss concept,source,characteristic,diagnosis,intervention of The Allergic constitution deeply.Supplement and consummate the theory system of the Allergic constitution;15 cases of the Allergic constitution and 30 cases of the Balanced constitution were selected by the standard of the Allergic constitution and the Blanced constitution.The regular physical examination,TCM symptoms and syndromes were investigated accordingly.Total RNA was extracted from peripheral blood,and it was amplified and labeled.The sample were checked by Sentrix(?) Human-6 Expression Bead Chip.The data was analyzed and the gene symbols with significant difference were searched in GENE BANK database.the gene differential expression profiles and correlated pathway were analyzed.Results:"Allergic constitution "has not been found in ancient literature of TCM.The constitutions of TCM bring new ideas to the recognition of the the Allergic constitution.it considered the emphasis of medical care to the Allergic constitution is not the sensitinogen but the human being who suffer from hypersensitivity disease.The results showed that the peripheral blood gene expression levels were different in the Allergic constitution Comparing with the Blanced constitution,There were 86 genes expression up-regulated and 111 genes expression down-regulated.Fos gene and IL-16 gene up-regulated among the total,the result have resemblance with the findings about fos gene and IL-16 gene recently;several gene have intimate relationship with the IL-4signaling pathway and p38 MAPK signalilng pathway,the findings is valuable for the learning of the Allergic constitution to TCM.Conclusions:The sought about prevention and cure to the Allergic constitution based on the theory of The constitutions of TCM.it benefits the prevention and treatment of hypersensitivity disease.The peripheral blood gene differential expression profiles in the Allergic constitution was found for the first time.Fos gene and IL-16 gene up-regulating and several gene having intimate relationship with the IL-4signaling pathway and p38 MAPK signalilng pathway maybe the characteristic of the Allergic constitution,...