Chemical Studies On The Efficacy Substance Of The Processing And Combination Of Fructus Corni

The chemical components in traditional Chinese medicine(TCM)are responsible for their therapeutic effects and very important in the modernization of TCM. A serious of systematic studies-the processing procedure,variety regulations before and after the processing,mass spectrometry characteristics of glycosides,chemical changes happened in the combinations of Fructus Corni with other five TCM common used in clinic, was carried on by using various modern analytical methods to provide theoretical basis and scientific methods for clarifing the processing principle,establishing the components database and mass spectrometry fingerprints,gauging the processing method and annotating the combination mechanisms.Standard substances of Fructus Corni in existence were firstly analyzed by using electrospray ionization tandem mass spectrometry (ESI-MSn) and Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) using sustained off-resonance irradation/collision-induced dissociation (SORI-CID) method at high mass resolution. Mass spectrometry character behavior has been summed up and product ion mass spectra have been examined at high mass resolution allowed unambiguous determination of the elemental composition of fragment ions. Both the hydrogen and deuterium exchange and the alkali metal cationization on fragmentation pathways in mass experiments can provide complementary useful structural information. These characteristics can be used to distinguish similar structure in glycoside compounds, organic acid compounds and their isomers simply and efficiently.By using HPLC and UV analysis methods, L9 (34) orthogonal designs were carried on for the optimizations of the ultrasonic extraction Procedure and the processing procedure of Fructus Corni. And then the moisture content, ash content, and the main effective constituents were determined and the corresponding quality standards were established which played an important role in the identification and quality control.The variety regulation of effective components in crude and processed Fructus Corni was investigated by using HPLC- ESI, ESI-MS and UV. The results show that there is an obvious change in the procedure of Fructus Corni processing, which agreed with the effect dissimilarity of crude Fructus Corni with processed Fructus Corni. Then the extracts of Frutus Corni have been analyzed by HPLC-ESI-MSn. According to these mass spectrometry characteristic regulations and the retention time (tR) of HPLC, the structures of seven kinds of glycosides have been identified. The characteristic fragment ions of CID of glycosides have been discussed, which are specific and useful for the identification of some glycosides in Fructus Corni.Guided by the Chinese medicine theories, the changes before and after the combinations Fructus Corni with different medicine herbs in different proportion were studied by using HPLC, ESI, etc. The experiment results show that the effective components in Frucus Corni are stable in acid and neutral solutions, but they could influence the constituents in other medicine materials. A part of changes occurred in the decoction. It provides chemical basis for studying traditional Chinese medicine prescription.