To Strengthen The Study Of Open-heart Surgery Myocardial Protection

Experimental study of protective effects of Fructose -1.6-Diphosphate (FDP) and Captopril(CAP) on myocardial ischemial reperfusion injury.utilizing the isolated perfused working rat heart model, the mechanism and protective effects of myocardial ischemial reperfusion injury on left ventricular function, myocardial ca^? content, lactic dehydrogenase[LDH) and myocardial ultrastructure were studied. There were six groups for study and 8 rat heart in each group in this experiment: (1) control group: Cardial arrest for 20 min under normothrmal(37±0.5℃) and reperfusion for 35 min; (2) Grorp of drug administration during the reperfusion: There Were two subgroup, in this groups, FDP₁(perfusate Contained FDP)and CAP₁(perfusate containced Captopril.) (3) Group of drug administration from the beginning of ischemia There were three subgroups in this group, namely FDP₂. CAP₂ and FDP₂ + CAP₂. In these subgroups the drugs above-mentioned added to the perfusate respectively.The results showed: In the control group, the cardiac function deteriorated significantly, the change of left ventricular function in diastolic phase was earlier and more obvious than that in the systolic phase. The myocardial ca^? content and the LDH release increased significantly, in the subgroup FDP, the index of left ventricle contraction were nective reaction. The Protective effecf of FDP on myocardium was lowere than that of CAP. in subgroup CAP₁ as well as in CAP₂. In these two CAP subgroups the significant protective effect on myocardium was observed and there was no significant difference between them. The more significant protective effect on myocardial uitrastructure. LDH resease and ca^? content appeared in the subgroup FDP₂ and the result in subgroup FDP₂ was better than that not only in subgroup FDP₁ but also in subgroup CAP₁ and CAP₂. Results indicated: 1. FDP and CAP could signficatly reduce the reperfusion injury, but, the much protective effect of FDP was only administered at the beginning of ischemia other than of reperfusion. Administration of FDP Combined With CAP could provide better Protective effects during the period of the ischemia. 2. myocardial reperfusion injury was related directly to the myocardial ischemis injury. Improving anoxia Could Play an important role in preventing reperfusion injury.