| Objective To investigate the effects of Parathyroid Hormone-Related Protein N-Terminal fragment(PTHrP1-34) at various administration dosage on biochemical markers of bone turnover,cytokines regulating bone remodeling,bone mineral density (BMD),bone histomorphometry and biomechanical properties in ovariectomized(OVX) rats in order to provide some theoretic evidence for using PTHrP1-34 treatment in osteoporosis and find its effective administration dosage.Methods 60 female Wistar rats of 4-month age were used in this study and 50 of them were ovariectomized and divided into 5 groups of Placebo , E2,PTHrP20, PTHrP 40 and PTHrP 80.Another 10 rats were Sham-operated and used as a control group (Sham).After five weeks ,PTHrP1-34 was subcutaneously injected once daily to the rats of PTHrP20, PTHrP40 and PTHrP80 groups with 20 40 80 μ g · kg-1 body weight respectively. The rats of Placebo and Sham groups were injected with 0.2ml saline once every 3 days . The E2 group rats were injected with estradiol benzoate at a dosage of 40ug · kg-1 once every 3 days. After treatment of 12 weeks the femora and lumbar vertebrae were removed for the determination of the BMD and bone biomechanical test, and the tibia metaphyseal bone was analyzed using conventional histomorphometry techniques,the femora was performed the three points bend test,the lumbar vertebrae was done the compressive test.Drawing the jugular vein blood to get the serum for testing the E2 Ca P ALP TNF-α IGF- I BGP content. Results 1. The BMD and bone strength of Placebo group were significantly...
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