Olfactory Dysfunction Resulting From Allergic Rhinitis In Mice And The Effects Of Glucocorticoids In Treating

Objective1. To obtain the incidence rate of olfactory dysfunction in allergic rhinitis.2. To observe the expression in olfactory mucosa of mice with olfactory dysfunction from allergic rhinitis. And to observe the expression of olfactory marker protein (OMP) in murine olfactory epithelium.3. To observe the effects of intranasal glucocorticoid(GC) and systemic GC on the expression of OMP in murine olfactory mucosa with allergic rhinitis.4. To investigate the different effects on the expression of OMP between intranasal and systemic GC. Try to find out the best route of administration.Methods1. An allergic rhinitis animal model was established by intraperitoneal injection and intranasal application of ovalubumin to mice.2. The function of the mice was evaluated by buried food test(BFT).3. The expression of OMP in the olfactory mucosa was tested by immunohistochamistry.4. After intranasal application of budesonide or intraperitoneal application of betamethasone, test the expression of OMP in olfactory mucosa at the7th day and the14th day.Results1. About75.3%of mice suffered from allergic rhinitis with olfactory dysfunction.2. The olfactory epithelium became thinner in mice with allergic rhinitis than control group. The down-regulation of OMP in olfactory epithelium was observed in mice with allergic rhinitis compared with control group. There was significant difference in the group with olfactory dysfunction(P<0.05) compared with control group, and no significantly different in group without olfactory dysfunction (P>0.05). 3. The up-regulations of OMP in olfactory epithelium were observed in budesonide groupl and betamethasone groupl compared with the group non-drugs (P<0.05).4. The expression of OMP in olfactory epithelium in budesonide group2was similar to budesonide groupl. While the expression of OMP in betamethasone group2was similar to betamethasone group1.Conclusion1. Olfactory dysfunction is a very common symptom in allergic rhinitis.2. Allergic rhinitis cause histologic changes in olfactory mucosa. It can down-regulate the expression of OMP in olfactory epithelium.3. Both routes of applications of GC can up-regulate the expression of OMP in olfactory mucosa. And the affects can keep a while.SummaryGlucocort icoids are the common used drugs in treating olfactory dysfunction, but the action mechanism is still poorly understood. Allergic rhinitis is an important factor of olfactory dysfunction not only cause of blockage but also cause of the change of mucosa by inflammation. Animal model was used to observe the expression of OMP in olfactory mucosa, especially after intranasal or systemic application GC In order to investigate the possible action mechanism of glucocorticoid for olfactory dysfunction and the protection action for the murine olfactory epithelium with allergic rhinitis.