The Observation And Experimental Study Of Moringa Oleifera Aqueous Extract On Anti-influenza A (FM1Strain)

Influenza A is the main pathogens, which cause viral acute respiratory disease and pandemic repeatedly in global world. The acute onset, high incidence and a wide range of prevalent are the major three characters of influenza A in pandemic. In the old and in the youth, it will cause complication disease during the co-infection, such as pneumonia. According to the data in records, there were three wide-ranging pandemic onsets in the world:the pandemic of Spain in1918, the pandemic of Asia in1957and the pandemic of Mexico in2009, which were cause large damage of economic with high mortality rate. Base on the above mentions, it is a focus task to seek an effective method to cure and prevent the influenza A.On clinical, the occur of the viral variation and the resistance to drugs are so rapid, which limit the usage of the western medicine in therapy. According to the fact of that, a new type of therapy method to anti-influenza A present. It is on the base of the Chinese traditional herb, combined with the Chinese tradition theory of diagnose and treatment. Lots of the Chinese traditional classical prescriptions are used in the therapy of influenza A infection, and get much positive effects. Nowadays, the therapeutic method of the combination western medicine with Chinese traditional herbs are accepted by more and more health care works, and which are get more recognition and development.Moringa oleifera, which is a common nature herb in India traditional medicine, is a novel popular health food in Europe. Moringa oleifera is originated in Africa and India, but in recent years, it has been widely planted in Guangdong, Yunnan and Hainan provinces in China. Moringa oleifera is pungent nature, tiny warm and have no toxin. Recent studies demonstrated its rich nutrition compositions, such as Vitamin A, Vitamin B, Vitamin C, protein, and a variety of minerals, it also contain the essential amino acids of human body. The whole plant of Moringa oleifera can be used in different ways. The root and the leaves of the plant can be used as herbs or spices, the fruit of the plant can be used as a flavor eatable vegetable, seeds of the plant can be used as a health food with rich oils or be used as raw material of spices and cosmetic. The powder of the beans is a water purification agent recommended by the International Environmental Organization. Following by the research of the composition of Moringa Oleifera, the scientist have great interesting in the bio-activities of this plant. The scientists found that Moringa oleifera had various bioactivities such as anti-bacterial, anti-inflammatory, hepatoprotective, anti-tumor, hypolipemia activities and so on. But on the anti-virus researching study, it is still lack of data to support.According to the above base literature research of Moringa Oleifera, we want to take some base studies into the anti-virus effect researching. Firstly, we examine the inhibition effect on common respiratory virus in vitro, including rhinovirus14, adenovirus7, respiratory syncytial virus, influenza A and influenza C. The results showed that the aqueous extract of Moringa oleifera had good effect to inhibit the influenza A and respiratory syncytial virus, but showed weak prohibit functions on rhinovirus and adenovirus with no-toxic concentration. The aqueous extra of the plant had no effect on anti-influenza C. In the further studying, we found that the aqueous extract of Moringa Oleifera could decline the CPE degree caused by the influenza A FM1strain on MDCK cells, inhibit the hem-adsorption lever in the chicken erythrocytes absorption test. Simultaneously, the aqueous extract of Moringa Oleifera could prohibit the viral proliferation by inhibition plaque-forming test and the transcription level of viral HA gene by RT-PCR. In vivo, the aqueous extract of Moringa Oleifera (concentration is20g/kg)could protect the mice from the viral infection and reduce the pathological degree of the viral pneumonia. It also could regulate the cellar factors of the body, by reducing the secreting level of IL-6, TNF-a and increasing the secreting level of IL-2andlFN-y.In summary, the primary studies showed that the aqueous extract of Moringa Oleifera had good effect on prohibit influenza A virus through inhibition of the viral proliferation and the transcription lever of HA gene, it also had effective function on enhancing the body immunity.