Effect Of Moringa Oleifera On Intestinal Length And Histomorphology In Mice

Short bowel syndrome(SBS) is a major cause of intestinal failure. This isa clinical syndrome whichis caused by extensive resection of the small intestine and characterized by a series phenotypes of diarrhea, weight loss and malnutrition. Currently, there is no effective treatment or medication with less side effects in clinical SBS. Therefore, it’s necessary to exploit an effective medicinal food in promoting intestinal growth, as a safe and effective treatment.Wefirstly preparednutrient-matched isocaloric control, 50 % MO and 100 % MO to feed.KM mice. In 65 d, MO feedhad a positive effect on mouse small intestinal growth. Thereafter, the pathological observation and RT-PCR testrevealed that Moringa fed mice resulted in a significant increase in the inner surface area ofthe small intestine. The results showed that 100% MO not only the length of the small intestine increased by an average of about 10 cm, but also the intestine villi length and jejunum perimeter respectively increased 14.4% and 69.3% in comparison to the control. As for intestinal epithelial cells, the anti-apoptotic related Il15, Bcl21, Bcl3,Mcl1 and tissue remodelingrelated Wnt2 b, Vegfa, Actb, Thbs1, allacquired higher relative mRNA levels in50% MO or 100% MO. At the same time, calorie intake, blood glucose, glucose tolerance,serum insulin, serum inflammatory cytokines, liver function, liver pathology structure and many other indicators were tested, to provide more comprehensive perspective for the overall data analysis and follow-up researches.Moringa oleifera Lam. is an affinal drug and diet,which usually effects on anti-oxidation, anti-bacterial, anti-tumor, anti-hypertensive and other significant aspects. For a long time, researchesare not deepon the efficacy and mechanism of Moringaactivity in gut. This paper identified the role of Moringa promoting intestinal growth, and preliminary explored its mechanism to lay a foundation for future study. Depth study of this subject will provide a new research orientationortherapeutic methodof SBS.