The Effect Of Chinese Medicine On Osseous Tissue In Periodontitis Rats

ObjectiveBuilding an experimental tooth movement animal model of periodontitis according with the treatment of traditional Chinese medicine,lays the foundation for the study of periodontitis orthodontic treatments of integrated traditional and western medicine.It can provide exact plans for periodontitis patients who need orthodontic treatments.MethodsUseing 0.20mm orthodontic wire ligate the cervical margin of SD rats maxillary molar, and with the method of high carbohydrate diet to establish periodontitis model of rats. Using the maxillary molar area ligation wire and the extension spring between maxillary anterior tooth and molar, connected to form a strength device of 50g, create orthodontic reason mobile conditions. By gross observation, oral examination and HE staining, we can evaluate the orthodontic movement of periodontitis rat model.80 SD rats were randomly divided into three groups, namely the control group (N,20 rats), periodontitis orthodontic group (OP,30 rats), periodontitis orthodontic treatment group (OPY,30 rats). After the periodontitis modeling was completed, gave a daily medicine gastric lavage to the OPY group, with the same volume of saline water to the other groups. Traditional Chinese medicine was Bazhen decoction, selecting astragalus, liquiritia glycyrrhiza, Angelica, white peony, baikal skullcap root, Poria cocos, the rhizome of chuanxiong, Drynaria. Through filtration and concentration, the medicine were administered to SD rats enlarging 15 times adult doses. Evaluate the orthodontic move-ment of each group by gross observation and oral examination after three weeks.Meanwhile, the influence of traditional Chinese medicine on periodontal inflammation in orthodontic tooth movement in rats was evaluated through the gingival index(GI), depth of pocket(DP) and tooth mobility(TM) assessment. ELISA assay of SD rats molar periodontal tissue interleukin 6(IL-6) content, after standardization of each group within IL-6 content, can evaluate the control function of this prescription for inflammation.For the influence of traditional Chinese medicine to the perio-dontitis rat bone remodeling during orthodontic movement, it is through the tooth spacing values, tooth mobility(TM), frontal resorption(FR) and periodontal modeling area organization osteocalcin (OC), trans-forming growth factorβ1 (TGF-β1) and osteoprotegerin(OPG) to evaluate the content.In order to observe the prescriptions for the quality of periodontitis in rats kidney physiological impact of orthodontic, we had chosen twelve months old female SD rats to established the kidney physiological impact orthodontic periodontitis model, with reference to the previous methods to evaluate the control group, model control group and treatment group situations.ResultsAfter build the SD rat model, GI, DP, TM and alveolar bone absorption value with the normal group were significantly different, the space between molar teeth and anterior teeth decrease above 2mm, the periodontitis rat orthodontic treatment model was successfully builded.In this study, according to syndrome differentiation of traditional Chinese medicine principle, the prescription is formed by the Bazhen decoction. The periodontitis orthodontic treatment group is distinctly superior through the evaluation of the oral examination, histological and immunological test (ELISA) observed each index (IL-6,TGF-β1,OC, OPG).The prescription can be good medicine control of inflammation and bone remodeling.Using the rat of kindney deficiency physique to establish an experimental tooth movement animal model of periodontitis,the result becomes more apparent with the same evaluation method.ConclusionLigation of the neck of molar teeth and high carbohydrate diet not only can destroy the periodontal structures, but also provide the conditions for the formation of periodontitis.It can promote the modeling methods of the SD rat periodontitis. Compared to hormone injection, it introduces less exogenous factors and has good reproducibility of the clinical treatment of inflammation and bone remodeling.The rats fed in traditional Chinese medicine of the prescription get a proper orthodontic tooth movement in the case of reduced periodontal tissue destruction.The four factors in periodontal tissue indicate that the traditional Chinese medicine can be a very good anti-inflammatory and beneficial treatment effect of bone remodeling during the process of orthodontic treatment model periodontitis in modeled rats.In the study of twelve months old female SD rats, the traditional Chinese medicine also has good effect in anti-inflammatory action and bone remodeling during the process of orthodontic movement. It has a significant difference between JN group and JY group, and the difference is greater than the rats of the normal physique. The prescription may be speculated that it has better clinical effect in elderly patients or patients of kindney deficiency physique.