Study Of Acupuncture On Immunoregulation In Allergic Rhinitis Rats

ObjectiveAllergic rhinitis(AR) is allergenically inflammatory disease of type I induced by allergen, which is by The method of IgE and is characteristic by The esophocyte, mast cells, T lymphocyte. The symptom is nasal itch, sneeze and snivel with The complication of asthma. The abroad study revealed the occur of allergenic nasatitis is closely related to economic development. Recent years, the mobidity have inceased significantly to 10% with serious damage to the health of PeoPle.AR is treated by modern medicine with immunal Therapy or durg, which have a lot of shortcoming. For immunal Therapy, The allergenic antigen is difficult to b3:determined, The effect is slow to occur, The course is too long to finish for the patients, The disease is difficult cured. The drug treating Allergenic nasatitis consist of durg anti-histamine, cortisol, atropine, with rapid effect and more side effect.AR is calle "biqiu", "qiuti" in Traditional Chinese Medicine. Acupuncture treats the AR with long time and good results. But the immunal mechanism is undermined. In our study, the immunal mechanism of Acupuncture treating the allergenic nasatitis in investigated, which Provide scientific (?)rmation for therapy. Methods1. Published Articles are StudiedThe Published artieles are sudtied to declare the clinical and experimental investigation and provide the prospection.2. Experimental Study(?) our study, the effects of Acupuncture on The AR are investigation in (?)vation, pathology, TLC4 in NLF, IL-4 in blood, TLR4 and NF-κB in Nose mucosal.2.140 SD rats, SPF and 180-220g weight are randomly divided into four groups:normal control group, model group, acupuncture treatment group and Fluticasone Propionate Nasal Spray(FPNS) treatment group.2.2 Rat model of allergic rhinitis was prepared by injecting egg album and stimulating nasal mucosa.2.3 Normal control group was not treated. acupuncture treatment group was treated by acupuncture on DU 14(Dazhui), BL 23(Shenshu) and BL 13(Feishu). FPNS group was treated by FPNS, model group was treated by physiological saline.2.4 The behaviors of rats in all groups were observed. The pathological changes of nasal mucosa were tested and the contents of TLC4 in NLF, IL-4 in blood, TLR4 and NF-κB in Nose mucosal were determined by radio-immunologic analysis.Results1. Compared with normal control group, the behaviors'scores of model group were significantly higher (p<0.05). After treatment, the behaviors' scores of acupuncture treatment group and FPNS group were significantly lower than those of model group (p<0.05)2. Compared with normal control group, the eosinophilic granulocyte(EOS) count of model group is significantly higher (p<0.05). After treatment, the EOS counts of acupuncture treatment group and FPNS group were significantly lower than those of model group (p<0.05)3. Compared with normal control group, the serum content of TLC4 in NLF and IL-4 in blood of model group is significantly higher (p<0.05). After treatment, the serum content of TLC4 in NLF and IL-4 in blood of acupuncture treatment group and FPNS group were significantly lower than those of model group (p<0.05)4. Compared with normal control group, the expression of TLR4 and NF-κB of model group is significantly higher (p<0.05). After treatment, the expression of TLR4 and NF-κB of acupuncture treatment group and FPNS group were significantly lower than those of model group (p<0.05)Conclusion1. Acupuncture treatment can effectively improve the symptoms of rats with allergic rhinitis. It means the method is effective for treating allergic rhinitis.2. The possible action mechanisms of Acupuncture treatment in treating allergic rhinitis may be as the follows::①It can significantly decrease the EOS count of nasal mucosa of rats with allergic rhinitis.②It can effectively decrease the serum content of TLC4 in NLF and IL-4 in blood of rats with allergic rhinitis.③It can effectively decrease the expression of TLR4 and NF-κB of rats with allergic rhinitis.