Studies For The Relationship Between The Experimental Allergic Rhinitis And Toll-Like Receptor 2 And Toll-Like Receptor 4

Part ⅠPreliminary Observation of Effect of LPS in ExperimentalAllergic RhinitisObjective: To observe the effect of LPS in allergic rhinitis. Methods: SD rats (n=40) were randomly divided into four groups: Allergic rhinitis model group B (n=10) were immunized by intraperitoneal injection of 200μg Ovalbumin (OVA) (1ml OVA-Al[OH]3-saline suspension) at day 1, 2, 3, and 11. At day 19, 0.1ml of saline containing 10mg of OVA was instilled into nasal cavity for 7 consecutive days. Normal control rats group A (n=10) were treated with the same methods except intraperitoneal injected and intranasal instilled only with saline. LPS administrated group C (n=10) were instilled LPS(10μg/0.1ml) into nasal cavity for 5 consecutive days. Allergic rhinitis model and LPS administrated group D (n=10) were challenged into allergic rhinitis, then were instilled LPS(10μg/0.1ml) into nasal cavity for 5 consecutive days. During the period of intranasal instillion, the symptoms, such as sneezing, itching and rhinorrhea, were observed. The nasal mucosae of them were studied routinely by HE and Toluidine blue staining to observe the infiltration of inflammatory cells. The numbers of eosinophils in the epithelium were counted under the light microscope. Results: 1.Animal model of allergic rhinitis was established by using ovalbumin intraperitoneal immunization and nasal challenge. 2.The symptom scores of Allergic rhinitis model group B and Allergic rhinitis model and LPS administrated group D were increased; Their scores were higher than those of normal control group A and LPS administrated group C (P<0.01). In the period of LPS administration (day 8-12), the scores of Group D were higher than those of the other groups (P<0.01). The scores between Group A and Group C were no significant differences (P>0.05). 3.The numbers of eosinophils in the epithelium of Allergic rhinitis model group B and Allergic rhinitis model and LPS administrated group D were increased; The numbers were higher than those of normal control group A and LPS administrated group C (P<0.01). The numbers of Group D were higher than thoseof Group B (P<0.05). The numbers between Group A and Group C were no significant differences (P>0.05). Conclusions: 1.The Animal model of allergic rhinitis has been made successfully; OVA is an economical and safe allergen. It can induce the animal model of allergic rhinitis in SD rats effectively. 2.LPS can exacerbate the symptoms and the pathologic changes in allergic rhinitis.PartⅡnStudies for the Relationship between Toll-like receptor 4 and Experimental allergic rhinitisObjective: Study the relationship between the toll-like receptor 4 and allergic rhinitis. Methods: The experimental animals and the groups were the same as part I . The expression of TLR4mRNA was detected by reverse transcription polymerasechain reaction (RT-PCR) in nasal mucosa and blood of all the groups. The nasal mucosae of them were studied by immunohistochemical staining to observe the TLR4 expression. Results: TLR4mRNA and TLR4 protein expressed in all the groups. And the gene expression of TLR4 in group B, group C and group D was significantly higher than that in group A (P<0.01). The gene expression showed no statistical differents between group B and group C (P>0.05). While, the gene expression in group D was significantly higher than that in group B and C (P<0.01). The intensity of positive immunoactivity of TLR4 in group B, group C and group D was significantly higher than that in the group A (P<0.01). The protein expression showed no statistical differents between group B and group C (P>0.05). While, the expression in group D was significantly higher than that in the group B and group C (P<0.05). Conclusions: 1 .TLR4 can express in nasal mucosa and blood of the rat. 2.It suggests that epithelia in nasal mucosa had protective and immune function, which could defense bacterial infection through Toll-like receptors. 3.The expression of TLR4 increased in nasal mucosa and blood of the experimental allergic rhinitis. It suggests that TLR4 have effect on allergic inflammation .