| BackgroundThe essential component of the famous "Yunnan Baiyao" are san qi (Panax notoginseng) and other Chinese Herbal Medicine, and it is already used widely in clinical almost one hundred years, and has unique, reliable curative effect especially in treating of bone fracture. But we know less about the biological Mechanism of action.At present, a lot of studies about Baiyao are stay at the observing stage of clinical effects, rarely study about the analysis of effective constituent and mechanism of action. And the study about pharmacology and pesticide effect of Compound Baiyao is Low levels and repeated. And short of systematic and Intensive study at molecular and cellular level, and lake of Good and modern basis of Pathophysiology.The Pathophysiologic study model is practically the same as Western medicine. So is unable to reflect principal clinical and pesticide effects of compound Baiyao objectively, and this is strategic point of to can make a breakthrough an indicator of at the study of compound Baiyao.ObjectiveAs our country Famous "Yunnan baiyao", it is necessary to intensive study the bone formation and mechanism of Yunnan Baiyao and major components on treating bone fracture by means of making external cells (MSCs) cultivation and further animal experiment with adopting the contemporary Molecular biology technique, to explore the mechanism of actions of Yunnan Baiyao and major components on promoting bone tissue repair.MethodsThis project, first, put a broken prescriptions study into practice by observing the Yunnan Baiyao and ten major components (code name) expect that proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) from SD rats and the express change of bone Matrix Protein and related growth factor. To explore the effect of Yunnan Baiyao and each component on that proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells and the cellular molecule mechanism of promoting the healing of bone fracture.Second, to look for efficient and harmfulless component from earlier studies, and design the new compound Baiyao application locally in the healing of rabbit bone fracture by fall together analyse and comprehensive. To clear and definite the located position and action of Yunnan Baiyao and each component, and explore the mechanism of promoting the healing of bone fracture through dynamically observe the situation of bone tissue repairing by detection of x-Ray, histology, immunohistochemistry, molecular biology and the effect of Yunnan Baiyao and each component on some kinds of cell factor and collagen during the repairing of bone fracture.Results1. Variety of Yunnan Baiyao components stimulate the differentiation of osteoblasts derived from bone marrow stromal dells by that facilitate the production of some factors such as TGF-β,BMP, and encourage the activity of osteoblast, advance the composition of bone matrix. E, G, H, I, J components have obvious effect on the differentiation of bone marrow stromal dells and have lower cytotoxicity in all of Yunnan components.2.One to six number new formulating Baiyao has obvious effect on promoting the healing of bone fracture by proliferation of endothelial cell and formation of new blood vessels in early and medium term during the repairing of rabbit bone fracture.3.This study find that Baiyao also have obvious effect on proliferation and differentiation of osteoblast by promoting combination of collagen I and integrainαβand activating FAK-way and formatiing local new blood vessels and improving condition of local oxygen supply. It is possible contribute to accelarte speed and quality and reduce the process of the healing of bone fracture by that new formulating Baiyao suppresed the process of cartilagenous and promoted that of intramembranous ossification.Conclusions 1. In early and medium term during the repairing of rabbit bone fracture, the possible mechanism of Yunnan Baiyao and major components in promoting bone healing was to directly or indirectly effect on the proliferation of BMSCs, enhance the activity of osteoblast, and the formation of new blood vessels, therefore, lead to blood supply plentiful and cell vigorous of fracture local.2. It is possible the Yunnan Baiyao and major components had same stimulative fouction similar as bone growth factor and vascular growth factor. Among all of components, E,G,H,I,J had obvious stimulative effect on the differentiation of bone marrow stromal dells and have lower cytotoxicity, which had foundation of farther research and development.3. The experimental result that the Compare pharmacological action of in each Baiyao component, new formulating Baiyao and compound Baiyao is complicated. Although the 1 to 6 number new formulating Baiyao has a common effect on promoting the healing of bone fracture, but this research did not show obvious relativity and Differentiation both between and within new formulating Baiyao components as we thought before. We did not understand that each Baiyao component play what kind of role in the compound Baiyao, and what is the specific active constituent in all of Baiyao components. Especially the definite constituent in which and the possible mechanism of promoting the healing of bone fracture need more intensive work to do.
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