The Study Of Yunnan Baiyao Interfere Porphyromonas Gingivalis Induces Bone Destruction

Objective:To establish the experimental animal model that was infected with Porphyromonas gingivalis (P.g). Evaluation of effects of Yunnan Baiyao interfere P.g induces bone destruction.Searching for the new way to use in the prevention and treatment of inflammation disease of bone and periodontitis.Methods:72 health male Kunming rats,50-day old,were randomly divided into three group:model group (group M=24),treatment with Yunnan Baiyao group (group Z=24) and control group(group N=24).The rats of group M and Z had been inoculated with P.g (1*108CFU/ml) 200ul at the midline of the scalp between the ears twice daily; group N got the equal volumn saline with the same position and methods. At the same day the established of model, group Z had gotten the gastric perfusion Yunnan Baiyao 0.5ml(12.5mg/ml) for each rat daily,while group M and N just doing gastric perfusion with saline 0.5ml.8 rats on each group were killed on the day of 5th,8th and 14th. HE staining was employed to detect the inflammation condition and bone destroy. ELISA was employed to detect the level of TNF-α,IL-6 in serum.TRAP was employed to calculate the number of osteoclast in calvaria. RT-PCR was employed to detect the expression of RANKLmRNA and OPGmRNA in calvaria. Statistical analysis had been done at the end.Result:Doing analysis of the level of TNF-a and IL-6,the number of osteoclast and the RANKLmRNA expression in the group M,Z and N on the day of 5th,8th and 14th. The data was apparently difference between group M and Z(p<0.05),and on the day of 5th the data was significant increase in group M;the data also demonstrated that group Z and M had increased contrast with group N (p<0.05).Doing analysis of the OPGmRNA expression in the group M,Z and N. The data in the group Z apparently higher than in the group M and N(p<0.05),and the biggest difference happened at the day of 8th.Compare group M with N,the data was lower in group M on the day of 5th(p<0.05),but it became higher than group N on the day of 5th(p<0.05) and 14th(p>0.05).Discussion:The results demonstrated that Yunnan Baiyao is effective to inhibit bone destruction and improve bone repair. That include decrease the level of TNF-a and IL-6 in serum, decrease the expression of RANKLmRNA,enhance the expression of OPGmRNA and inhibit the production of osteoclast.