The Research Of Molecular Mechanism About Relating Neuropeptide Gene Expression And Immune Function Of Immunosuppressive Rats By Means Of Different Acupuncture Points

Objective To observe the effect of different acupuncture points in immunosuppressive rats on the neuropeptide gene expression,e-xplore the immu ne regulatory me chanisms,to clarify role of ac-upuncture on the neuroendocrine-immune network.Methods Preparation immunosuppressive rats model by intrape-ritoneal injection of CP .To observedβ-EPmRNA, SPmRNA exp-ression changes in brain tissue of rats by RT-PCR after acupun-cture;observed spleen cell surfaceβ-EPR and SPR expression us-ing western-blot; by FCM observed CD4+T cells and CD8+ T c-ells in blood; using ELISA method to observed IL-4, IFN-γlev-els of serum.Results Different points acupuncture can improveβ-EPmRNA,SPmRNA expression in immunosuppressive rats, compared to mod-el group,there is a significant difference (P<0.05), E group is t-he best. Acupuncture can reduce SPR expression on the splenic lymphocytes surface,improveβ-EPR content; different points acu-puncture can improve peripheral CD4+T cells content , compare-d to model group, there is a significant difference (P<0.05).CD8+T cells had no significant difference. Different points acupuncture can significantly decrease serum IL-4, increase serum IFN-γcontent of immunosuppressive rats, compared to model group,there is a significant difference (P<0.05),E group is the best.Conclusions Acupuncture can improve the cellular-immune funct-oin of i mmunos uppressi ve rats, particular ly the head plus body acupuncture points(E group) is best. The possible mechanis m is that affects neuropeptide-immune cell receptors-cytokins pathway.